Role of astrocyto in enteric nervous system and effect of anesthetic on enteric nervous system

• Project/Area Number: 15K10548
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: Aichi Medical University
• Principal Investigator: Hashimoto Atsushi 愛知医科大学, 医学部, 助教 (00378035)
• Co-Investigator(Kenkyū-buntansha): 木下 浩之 愛知医科大学, 公私立大学の部局等, 客員研究員 (70291490)
• Co-Investigator(Renkei-kenkyūsha): Feng Guo-Gang 愛知医科大学, 薬理学, 講師 (70351111)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 局所麻酔薬 / ヒト神経芽細胞腫 / 細胞死 / T型カルシウムチャネル / ヒト神経芽腫細胞 / パッチクランプ / 消化管筋間神経叢 / イレウス

Outline of Final Research Achievements

Local anesthetic, bupivacaine, is known to have neurotoxicity. Bupivacaine decreased viability of human neuroblastoma cell line (SH-SY5Y cell). T-type calcium channel blocker exacerbated the bupivacaine induced cell death. According to the result of realtime PCR, Cav3.1 was the only subtype of T-type calcium channel which expressed on the SH-SY5Y cell.

Research Products

• [Journal Article] Airway obstruction caused by acute transient thyroid swelling following fine needle aspiration biopsy of thyroid gland (2018)
  • Author(s): 橋本 篤、木下 浩之、磯部 英男、榊原 健介、藤田 義人、畠山 登、藤原 祥裕
  • Journal Title: Journal of the Japanese Society of Intensive Care Medicine Volume: 25 Issue: 3 Pages: 195-196
  • DOI: 10.3918/jsicm.25_195
  • NAID: 130006731115
  • ISSN: 1340-7988, 1882-966X
  • Year and Date: 2018-05-01
• [Journal Article] Advances in Ultrasound-guided Nerve Block：Sacral Plexus and Sciatic Nerve Block (2018)
  • Author(s): 橋本 篤、藤原 祥裕
  • Journal Title: THE JOURNAL OF JAPAN SOCIETY FOR CLINICAL ANESTHESIA Volume: 38 Issue: 1 Pages: 119-122
  • DOI: 10.2199/jjsca.38.119
  • NAID: 130006464097
  • ISSN: 0285-4945, 1349-9149
• [Journal Article] The Unknown Mechanism of Exogenous Tetrahydrobiopterin in the Renal Protection of Sheep Ischemia and Reperfusion (2018)
  • Author(s): Kinoshita Hiroyuki、Otake Kazunobu、Yamasaki Takashi
  • Journal Title: Anesthesia & Analgesia Volume: 126 Issue: 3 Pages: 1088-1088
  • DOI: 10.1213/ane.0000000000002784
  • Peer Reviewed
• [Journal Article] The unique action of nicorandil on cerebral circulation (2018)
  • Author(s): Kinoshita Hiroyuki、Kawahito Shinji、Takaishi Kazumi
  • Journal Title: Journal of Anesthesia Volume: 32 Issue: 3 Pages: 1-1
  • DOI: 10.1007/s00540-018-2499-3
  • Peer Reviewed
• [Journal Article] Cardiac arrest caused by rapidly increasing ascites in a patient with TAFRO syndrome: a case report (2017)
  • Author(s): Okumura Masatoshi、Ujiro Atsushi、Otsuka Yasunori、Yamamoto Hiroshi、Wada Sho、Iwata Hirofumi、Kan Toshiaki、Miyauchi Seiji、Hashimoto Atsushi、Sato Yuko、Fujita Yoshihito、Fujiwara Yoshihiro、Shimaoka Hideki
  • Journal Title: Acute Medicine & Surgery Volume: 4 Issue: 3 Pages: 344-348
  • DOI: 10.1002/ams2.278
  • Peer Reviewed / Open Access
• [Journal Article] Human serum albumin and oxidative stress in preeclamptic women and the mechanism of albumin for stress reduction (2017)
  • Author(s): Kinoshita Hiroyuki、Watanabe Kazushi、Azma Toshiharu、Feng Guo-Gang、Akahori Takahiko、Hayashi Hisaki、Sato Motohiko、Fujiwara Yoshihiro、Wakatsuki Akihiko
  • Journal Title: Heliyon Volume: 3 Issue: 8 Pages: e00369-e00369
  • DOI: 10.1016/j.heliyon.2017.e00369
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of superoxide generated by NADPH oxidase in the shedding of procoagulant vesicles from human monocytic cells exposed to bupivacaine (2017)
  • Author(s): Azma Toshiharu、Ogawa Saori、Nishioka Akira、Kinoshita Hiroyuki、Kawahito Shinji、Nagasaka Hiroshi、Matsumoto Nobuyuki
  • Journal Title: Journal of Thrombosis and Thrombolysis Volume: 44 Issue: 3 Pages: 341-354
  • DOI: 10.1007/s11239-017-1531-z
  • Peer Reviewed / Open Access
• [Journal Article] Phosphatidylinositol 3-kinase inhibition induces vasodilator effect of sevoflurane via reduction of Rho kinase activity. (2017)
  • Author(s): Miyamoto Y, Feng GG, Satomi S, Tanaka K, Fujiwara Y, Kinoshita H.
  • Journal Title: Life Sci. Volume: 177 Pages: 20-26
  • DOI: 10.1016/j.lfs.2017.04.005
  • Peer Reviewed
• [Journal Article] Does Memory Consolidation by Anesthetics Relate to a Time Window of Age? (2017)
  • Author(s): Kinoshita Hiroyuki
  • Journal Title: Anesthesia & Analgesia Volume: 125 Issue: 1 Pages: 357-357
  • DOI: 10.1213/ane.0000000000002163
  • Peer Reviewed
• [Presentation] Continuous rectus sheath block provides effective analgesia after breast reconstruction with free abdominal flap (2017)
  • Author(s): Atsushi Hashimoto, Hiroko Takayanagi, Haruna Kanamori, Yuko Sato, Yoshihito Fujita, Yoshihiro Fujiwara
  • Organizer: Anesthesiology 2017
  • Int'l Joint Research