| Project/Area Number |
15K10550
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology
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| Research Institution | International University of Health and Welfare (2016-2018)
Kawasaki Medical School (2015) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
千葉 義彦 星薬科大学, 薬学部, 教授 (00287848)
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 肺移植 / 肺 / 虚血再潅流傷害 / マイクロRNA / 麻酔薬 / RhoA/Rho-kinase |
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| Outline of Final Research Achievements |
We used lung samples from rat pulmonary ischemic reperfusion (IR) injury model. With western blotting, expression of RhoA and ROCK2 significantly increased in comparison with a control group. Expression of miR-133a-3p significantly decreased with real-time RT-PCR. Sevoflurane inhibited these expressions, suggesting that anesthetics inhibit the IR-induced airway hypersensitivity. Results of GO Slim function classification and pathway analysis about 153 genes increased DNA array suggested that IR injury is caused relatively in a short time with dynamically changes of genes.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究の目的は肺移植術後に気道過敏性が亢進するメカニズムを解明することである。ラット肺虚血再潅流（IR）傷害モデルを用いてWestern blot法、リアルタイムRT-PCR法を行いmiR-133a-3pおよびそのターゲットであるRhoAタンパク質が気道過敏性亢進の機序であることを解明した。またこの機序は吸入麻酔薬セボフルランで抑制され、麻酔薬が肺移植術の気道過敏性亢進を抑制することを示唆した。DNAアレイによる網羅的解析により各種遺伝子の発現がダイナミックに変動しIR傷害がもたらされる可能性を示唆した。
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