Analysis of mechanisms of neuropathic pain using in vitro nerve injury model
Project/Area Number |
15K10561
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Anesthesiology
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Research Institution | Kyoto Prefectural University of Medicine |
Principal Investigator |
HOSOKAWA Toyoshi 京都府立医科大学, 医学(系)研究科(研究院), 教授 (80165555)
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Co-Investigator(Kenkyū-buntansha) |
上野 博司 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (20381965)
原田 秋穂 京都府立医科大学, 医学部附属病院, 専攻医 (80433259)
深澤 圭太 京都府立医科大学, 医学(系)研究科(研究院), 助教 (90398404)
柴崎 雅志 京都府立医科大学, 医学(系)研究科(研究院), 助教 (20405319)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 神経障害性疼痛 / コンパートメントカルチャー / マイクログリア / 一次知覚神経 / コンパートメント培養 |
Outline of Final Research Achievements |
In vivo and in vitro neuropathic models were used to investigate the mechanisms of neuropathic pain. SNI model was used as in vivo model. Mechanical thresholds was dramatically decreased from day 1 after SNI procedure, and was kept on day 14 after SNI. Immunohistochemical analysis revealed that the accumulation of Iba-1 positive cells in both dorsal and ventral horn consisted with nerve injury from day 3 after SNI. Neurons dissected from dorsal root ganglion of mice were cultured using Campenot chamber in vitro model. Axons growth in the central compartment of Campenot chamber were observed, however those were not observed in both side compartments.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] 脳腫瘍による難治性頭痛に対してオピオイドが有効であった2症例とその機序についての考察.2015
Author(s)
大西佳子,細川豊史,坪倉卓司,深澤圭太,上野博司,権哲,原田秋穂,深澤まどか,山代亜紀子,谷口彩乃,波多野貴彦,田中萌生,仲宗根ありさ,岡田恵.
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Journal Title
Palliative Care Research
Volume: 10(2)
Pages: 509-513
NAID
Related Report
Peer Reviewed / Open Access
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