Functional analysis of the prostate cancer-related genes obtained from the new tissue culture system
Project/Area Number |
15K10580
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Urology
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Research Institution | Mie University (2017) Yokohama National University (2015-2016) |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
白石 泰三 三重大学, 医学系研究科, 客員教授 (30162762)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 前立腺癌 / 3次元培養 / 抗癌剤 / KIF22 / スフェロイド / 遺伝子 / 機能的解析 |
Outline of Final Research Achievements |
In our previous study, the prostate cancer-related genes were identified in the new tissue culture system. Among these genes showing enhanced expression in the new tissue culture system, the KIF22 gene was extracted as one of candidate genes related with prostate cancer behavior. In this study, we analyzed proliferation, apoptosis, and cell cycle of the prostate cancer cells (DU-145 and LNCaP) treated with KIF22 siRNA. The inhibition of KIF22 mRNA using siRNA affected cell proliferation in spheroids and monolayers of both cancer cell lines. This suppression also caused G2/M arrest and apoptosis in both cell lines. These results suggest involvement of the KIF22 gene in prostate cancer behavior.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Molecular mechanism responsible for fibronectin-controlled alterations in tissue stiffness in advanced chronic liver fibrogenesis.2016
Author(s)
(107)A.Iwasaki, K.Sakai, K.Moriya, T.Sasaki, D.R.Keene, R.Akhtar, T.Miyazono, S.Yasumura, M.Watanabe, S.Morishita, T.Sakai.
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Journal Title
J.Biol.Chem.
Volume: 291
Issue: 1
Pages: 72-88
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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