The exploration of new molecular markers in urine derived from prostate cancer patients by peptidomics

• Project/Area Number: 15K10587
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Kyoto University
• Principal Investigator: INOUE Takahiro 京都大学, 医学研究科, 准教授 (80511881)
• Co-Investigator(Kenkyū-buntansha): 松井 喜之 京都大学, 医学研究科, 助教 (00582107) ; 岡田 能幸 京都大学, 医学研究科, 助教 (60739291) ; 小川 修 京都大学, 医学研究科, 教授 (90260611)
• Research Collaborator: NAKAYAMA Kenji 京都大学, 医学研究科, 研究員 (30442594)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: 前立腺癌 / 診断 / 質量分析 / PSA / 前立腺 / MALDI / iTRAQ / 前立腺がん

Outline of Final Research Achievements

We have previously reported that a C-terminal PSA fragment composed of 19 amino acid residues (YTKVVHYRKWIKDTIVANP) with 2331 Da was statistically more abundant in post-digital examination urine of prostate cancer patients compared with in that of benign prostate hypertrophy patients by using MALDI-DIT-TOF/MS. In this study we have constructed quantitative analytical methods of the peptide using commercially available MALDI TOF/MS equipment. We have also evaluated expression of the peptide in prostate cancer cell lines and prostate cancer human tissues and finally detected in prostate cancer tissues but not in prostate cancer cell lines. In order to evaluate molecular markers for castration-resistant prostate cancer, we used iTRAQ method for comprehensive analysis of proteins expressed in LNCaP and AILNCaP cells. Up-regulated proteins in AILNCaP cells belongs to neuronal projection development and oxidation-reduction process by analysis of STRING database.

Research Products

• [Presentation] Identification and Characterization of Antioxidant Additives Dissolved from Polypropylene Plastic Tubes by MALDI-TOF/MS. (2018)
  • Author(s): Xin Li, Kenji Nakayama, Takahiro Inoue, Takayuki Goto, Shusuke Akamatsu, Osamu Ogawa
  • Organizer: 第2回京都生体質量分析研究会シンポジウム
• [Presentation] LCMSを用いた前立腺癌細胞株のリン脂質発現解析 (2018)
  • Author(s): 岡所 広祐、井上 貴博、中山 憲司、李 新、水野 桂、住吉 崇幸、牧野 雄樹、後藤 崇之、赤松 秀輔、小林 恭、小川 修
  • Organizer: 第27回泌尿器科分子細胞研究会
• [Presentation] 前立腺癌細胞株モデルを用いた去勢抵抗性癌への変異に関わるタンパク質の網羅的解析 (2017)
  • Author(s): 中山憲司、甲斐力、井上貴博、宮崎有、岡所広祐、小川修
  • Organizer: 第一回京都生体質量分析研究会シンポジウム
  • Place of Presentation: 京都 芝蘭会館
• [Presentation] 慢性腎臓尿中マーカーの開発を目指した疾患特異的な病的分子の探索ならびに化学修飾の検討 (2017)
  • Author(s): 神吉智子、桒原孝成、梅本周朗、藤本大介、水本輝彦、早田学、中山憲司、宮崎有、井上貴博、高谷宗男、小川修、向山政志
  • Organizer: 第一回京都生体質量分析研究会シンポジウム
  • Place of Presentation: 京都 芝蘭会館
• [Presentation] 前立腺マッサージ後の尿中PSA濃度の検討 (2015)
  • Author(s): 宮崎有、中山憲司、井上貴博、牧野雄樹 、植垣正幸、後藤崇之、岡田能幸、小林恭、寺田直樹、山崎俊成、松井喜之、神波大己、小川修
  • Organizer: 第74回日本癌学会学術総会、2015年10月8日、名古屋
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Presentation] Discovery of Urinary Biomarker Candidates for Prostate Cancer Based On Proteomic analyses (2015)
  • Author(s): Yu Miyazaki, Kenji Nakayama, Sadanori Sekiya, Takahiro Inoue, Takayuki Goto, Naoki Terada, Yuki Makino, Masayuki Uegaki, Yoshiyuki Okada, Takashi Kobayashi, Shigeki Kajihara, Shin-ichiro Kawabata, Shinichi Iwamoto, Tomomi Kamba, Koichi Tanaka and Osamu Ogawa
  • Organizer: 13th Urological Association of Asia Congress 2015
  • Place of Presentation: Shanghai, China
  • Year and Date: 2015-09-03
• [Presentation] 前立腺マッサージ後の尿中PSA濃度の検討 (2015)
  • Author(s): 宮﨑有、井上貴博、牧野雄樹、植垣正幸、後藤崇之、吉川武志、岡田能幸、小林恭、寺田直樹、山崎俊成、松井喜之、神波大己、小川修
  • Organizer: 第103回日本泌尿器科学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2015-04-18
• [Remarks] 京都大学医学研究科泌尿器科学教室（研究の概要―基礎的な研究―1)前立腺癌研究）
  • URL: http://www.urology.kuhp.kyoto-u.ac.jp/medic/research/overview/overview.html#1