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去勢抵抗性前立腺癌におけるWnt signalの生物学的意義

Research Project

Project/Area Number 15K10596
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Urology
Research InstitutionKumamoto University

Principal Investigator

河野 吉昭  熊本大学, 大学院生命科学研究部(医), 講師 (30593793)

Co-Investigator(Kenkyū-buntansha) 江藤 正俊  九州大学, 医学(系)研究科(研究院), 教授 (90315078)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Discontinued (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords前立腺癌 / Wntシグナル / β-catenin / 細胞増殖 / 抗アポトーシス
Outline of Annual Research Achievements

前年度にドキシサイクリン誘導性にβ-catenin shRNAが発現されるTR-βi細胞を用いたDNA microarray解析により、β-cateninノックダウンによる遺伝子発現低下を認めた分子Aに着目して解析を進めた。まず分子Aがβ-cateninの直接の標的遺伝子であることを確認するために、分子Aのpromotor領域を含むluciferaseコンストラクトをβ-catenin発現ベクターとともに293細胞に導入してレポーターアッセイを行なったが、レポーター活性の上昇を認めなかった。理由として、β-catenin/TCF複合体が結合部位がコンストラクトに用いたpromotor配列の外側に存在することが考えられた。また、β-catenin-shRNA発現ベクターと分子A発現ベクターを種々の組み合わせでLNCaP細胞に導入し、colony formation assayを行なった。β-catenin-shRNA発現ベクターによりcolony formationの抑制を認めたが、分子A発現ベクターの共発現によるcolony formation抑制の回復を認める結果には至らなかった。

Report

(2 results)
  • 2016 Annual Research Report
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2016 2015 Other

All Int'l Joint Research (4 results) Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 2 results) Presentation (2 results)

  • [Int'l Joint Research] CIC Biogune(スペイン)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Imperial College London(英国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] CIC Biogune(スペイン)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] Imperial College London(英国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Prognosis of metastatic renal cell carcinoma with first-line interferon-α therapy in the era of molecular-targeted therapy.2016

    • Author(s)
      Kawano Y, Takahashi W, Eto M, Kamba T, Miyake H, Fujisawa M, Kamai T, Uemura H, Tsukamoto T, Azuma H, Matsubara A, Nishimura K, Nakamura T, Ogawa O, Naito S.
    • Journal Title

      Cancer Science

      Volume: 107 Issue: 7 Pages: 1013-1017

    • DOI

      10.1111/cas.12951

    • NAID

      120005822782

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CXCL10 and CCL2 mRNA expression in monocytes is inversely correlated with the HLA-DR lower fraction of monocytes in patients with renal cell carcinoma.2016

    • Author(s)
      Motoshima T, Komohara Y, Horlad H, Tsukamoto H, Fujita M, Saito Y, Tanoue K, Kasejima Y, Sugiyama Y, Kawano Y, Nishimura Y, Takeya M, Eto M
    • Journal Title

      Oncol Lett

      Volume: 11 Issue: 3 Pages: 1911-1916

    • DOI

      10.3892/ol.2016.4132

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Everolimus (RAD001) sensitizes prostate cancer cells to docetaxel by down-regulation of HIF-1α and sphingosine kinase 1.2016

    • Author(s)
      Alshaker H, Wang Q, Kawano Y, Arafat T, Böhler T, Winkler M, Cooper C, Pchejetski D.
    • Journal Title

      Oncotarget

      Volume: 7 Issue: 49 Pages: 80943-80956

    • DOI

      10.18632/oncotarget.13115

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Phase II clinical trial of sorafenib plus interferon-alpha treatment for patients with metastatic renal cell carcinoma in Japan.2015

    • Author(s)
      Eto M, Kawano Y, Hirao Y, Mita K, Arai Y, Tsukamoto T, Hashine K, Matsubara A, Fujioka T, Kimura G, Shinohara N, Tatsugami K, Hinotsu S, Naito S
    • Journal Title

      BMC Cancer

      Volume: 15 Issue: 1 Pages: 667-667

    • DOI

      10.1186/s12885-015-1675-1

    • NAID

      120005758243

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sorafenib enhances the anti-tumor effect of anti-CTLA-4 antibody by inhibiting myeloid-derived suppressor cells.2015

    • Author(s)
      Motoshima T, Komohara Y, Horlad H, Takeuchi A, Maeda Y, Tanoue K, Kawano Y, Harada M, Takeya M, Eto M
    • Journal Title

      Oncol Rep

      Volume: - Issue: 6 Pages: 2947-2953

    • DOI

      10.3892/or.2015.3893

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] C5aR is frequently expressed in metastatic renal cell carcinoma and plays a crucial role in cell invasion via the ERK and PI3 kinase pathways.2015

    • Author(s)
      Maeda Y, Kawano Y, Wada Y, Yatsuda J, Motoshima T, Murakami Y, Kikuchi K, Imamura T, Eto M.
    • Journal Title

      Oncology Reports

      Volume: 33 Issue: 4 Pages: 1844-1850

    • DOI

      10.3892/or.2015.3800

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 前立腺癌細胞におけるWnt/beta-catenin-Survivin axisの生物学的意義2015

    • Author(s)
      河野吉昭、中田小百合、入江 厚、元島崇信、前田喜寛、鹿瀬島裕、今村隆寿、Robert Kypta、江藤正俊
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report
  • [Presentation] 前立腺癌細胞におけるWnt/beta-catenin-Survivin axisの生物学的意義2015

    • Author(s)
      河野吉昭 前田喜寛 元島崇信 鹿瀬島裕 杉山豊 江藤正俊
    • Organizer
      第103回日本泌尿器科学会総会
    • Place of Presentation
      ANAクラウンプラザホテル金沢
    • Year and Date
      2015-04-18
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-01-16  

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