| Project/Area Number |
15K10623
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
小林 憲市 滋賀医科大学, 医学部, 助教 (40727434)
影山 進 滋賀医科大学, 医学部, 講師 (50378452)
河内 明宏 滋賀医科大学, 医学部, 教授 (90240952)
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| Co-Investigator(Renkei-kenkyūsha) |
Akeo Hagiwara 同志社大学, 生命医科学部, 教授 (90198648)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 膀胱再生 / 泌尿器再建 / 足場材料 / 羊膜 / 口腔粘膜 |
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| Outline of Final Research Achievements |
The aim is to regenerate an autologous functional bladder tissue using amnion sheets and bioabsorbable scaffold with poly-lactic acid and poly-caprolactone copolymer P (LS/CL).
We cultured cells from oral buccal mucosa and stomach smooth muscle cells on the amnion and P(LS/CL) sheets. Moreover, epithelium (amnion sheet), middle layer(omentum), and muscle layer P(LS/CL) sheets were piled on subsequently, and wrapped by omentum for 3 weeks. Immunohistochemical studies showed that the epithelium was positive for cytokeratin and muscle layer was positive for α-SMA. Next we augmented regenerative bladder tissue onto native bladder with sufficient blood supply from omentum according to Clam method. After 6 months observation there were no complications, cystoscopic finding and cystography showed normal shaped bladder with normal peristalsis. These results indicated successful regeneration of functional bladder tissue using autologous tissues
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