Elucidation of selective renal progenitor differentiation and renal regeneration mechanism using cell sorting system
Project/Area Number |
15K10629
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Urology
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Research Institution | Nagoya City University |
Principal Investigator |
Nakane Akihiro 名古屋市立大学, 大学院医学研究科, 研究員 (70464568)
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Co-Investigator(Kenkyū-buntansha) |
西尾 英紀 名古屋市立大学, 大学院医学研究科, 研究員 (10621063)
林 祐太郎 名古屋市立大学, 大学院医学研究科, 教授 (40238134)
丸山 哲史 名古屋市立大学, 大学院医学研究科, 高度医療教育研究センター教授 (50305546)
水野 健太郎 名古屋市立大学, 大学院医学研究科, 講師 (70448710)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 再生医療 / セルソーティング / 腎臓 |
Outline of Final Research Achievements |
Pax2 transgenic ES cells were differentiated under the condition of addition of activin A and retinoic acid. Expression of various genes was evaluated by PCR method, and expression of BMP 7, Ret, Pax 8, aquaporin-1, and Podocin was enhanced. Expression of aquaporin-1 was also confirmed in immunostaining. EBs expressing the Pax2 gene was double-labeled with Pax2 and aquaporin-1 and cell sorting was carried out.It was possible to confirm increase of positive cells of Pax2 and aquaporin-1. From the results of this study, It was considered that the constitution ratio of some renal constituent cells or progenitor cells increased, and differentiation from ES cells to renal cells was induced by expressing the Pax2 gene.
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Report
(4 results)
Research Products
(11 results)
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[Presentation] Natural history and management of perinatally detected hydronephrosis, through long-term follow up.2016
Author(s)
Akihiro Nakane, Hayashi Yutaro, Mizuno Kentaro, Nishio Hidenori, Moritoki Yoshinobu, Kamisawa Hideyuki, Kurokawa Satoshi, Maruyama Tetsuji, Yasui Takahiro
Organizer
Society for Pediatric Urology’s 64st Annual Meeting
Place of Presentation
San Diego, CA, (USA)
Year and Date
2016-05-06
Related Report
Int'l Joint Research
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[Presentation] Comparison of robot-assisted, laparoscopic, and open pyeloplasty with a minimally invasive incision in children2016
Author(s)
Nakane Akihiro, Mizuno Kentaro, Hayashi Yutaro, Nishio Hidenori, Moritoki Yoshinobu, Kamisawa Hideyuki, Kurokawa Satoshi, Maruyama Tetsuji, Yasui Takahiro
Organizer
The 31th Annual Congress of the European Association of Urology
Place of Presentation
Munich(Germany)
Year and Date
2016-03-11
Related Report
Int'l Joint Research
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