Analysis of pathological stress control mechanism in kidney tissue stem cells

• Project/Area Number: 15K10641
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Okayama University
• Principal Investigator: Araki Moto 岡山大学, 大学病院, 講師 (90467746)
• Co-Investigator(Kenkyū-buntansha): 渡部 昌実 岡山大学, 大学病院, 教授 (70444677) ; 定平 卓也 岡山大学, 大学病院, 助教 (20733322) ; 植木 英雄 岡山大学, 医学部, 技術専門職員 (90537218)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 腎移植 / 病的ストレス / 腎幹細胞 / 分化

Outline of Final Research Achievements

We have established “Renal tissue stem cells that lead to regeneration of kidney corpuscle and tubules responsible for kidney function” in human cells. In this study, we aimed to elucidate the pathological stress control mechanism of kidney tissue stem cells. We selected renal ischemia and reperfusion injury in renal transplantation and acute renal failure as pathological stress.
We established "renal tissue stem cells" derived and separated from mice based on two new technologies we developed independently:" retrograde stem cell induction method" and "tissue specific stem cell separation method". We will establish “Renal tissue stem cells” in mouse cells. We are doing experiment using human renal tissue stem cells as well.

Academic Significance and Societal Importance of the Research Achievements

我々は既にヒト細胞では「腎機能を担う腎小体・尿細管の再生につながる腎組織幹細胞」を樹立している。しかしながら現時点でこの細胞治療が腎不全の根治的治療となるのは困難である。よって今回その技術を腎虚血再灌流障害という病的ストレスにおける腎臓再生能力の研究を行っている。腎虚血再灌流は腎移植・急性腎不全における主たる病的ストレスである。よってこの研究は新たな治療に結びつく可能性が高くその意義は大きい。