Genome-wide analysis for human spermatogenesis
| Project/Area Number |
15K10642
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
SHIRAISHI Koji 山口大学, 大学院医学系研究科, 准教授 (00535255)
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| Co-Investigator(Renkei-kenkyūsha) |
OHKAWA Yasuyuki 九州大学, 生体防御医学研究所, 教授 (80448430)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 男性不妊 / 網羅的遺伝子解析 / 精巣 / 非閉塞性無精子症 / 無精子症 |
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| Outline of Final Research Achievements |
Transcriptome analysis using next-generation sequencer (NGS) for human testicular samples has shown that data from 23,616 genes were available and gene ontology analysis has shown that the gene clusters for cell cycle, nucleic acid binding. Expression of human specific histone H3.5 was revealed to be decreased in men with non-obstructive azoospermia (NOA) compared with that from normal spermatogenesis. Immunohistochemistry using an antibody against histone H3.5 has shown that histone H3.5 plays roles on DNA synthesis in spermatogonia and spermatocyte. Transcriotpme analysis has also shown that expressions of cell cycle-related molecules in NOA men with varicocele and low expressions of these cluster of molecules was shown to be varicocelectomy-resistant cases. Transcriptome analysis could provide a novel informations for human spermatogenesis and open diagnostic and therapeutic windows for male infertility treatment.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Histone H3.5 forms an unstable nucleosome and accumulates around transcription start sites in human testis.2016
Author(s)
Urahama T, Harada A, Maehara K, Horikoshi N, Sato K, Sato Y, Shiraishi K, Sugino N, Osakabe A, Tachiwana H, Kagawa W, Kimura H, Ohkawa Y, Kurumizaka H.
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Journal Title
Epigenetics Chromatin
Volume: 9
Issue: 1
Pages: 1-16
DOI
Related Report
Peer Reviewed / Open Access
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