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Elucidation of signal transduction mechanism in the testis and its application to trreatment of male infertility.

Research Project

Project/Area Number 15K10645
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Urology
Research InstitutionNagoya City University

Principal Investigator

Umemoto Yukihiro  名古屋市立大学, 大学院医学研究科, 教授 (80381812)

Co-Investigator(Kenkyū-buntansha) 窪田 裕樹  名古屋市立大学, 大学院医学研究科, 研究員 (10347403)
佐々木 昌一  名古屋市立大学, 大学院医学研究科, 研究員 (50225869)
水野 健太郎  名古屋市立大学, 大学院医学研究科, 講師 (70448710)
岩月 正一郎  名古屋市立大学, 医学(系)研究科(研究院), 研究員 (70595397)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords男性不妊症 / Sertoli細胞 / Leydig細胞 / 遺伝子導入 / SCO / anti Mullerian hormone / パーコール法 / Sertoli細胞 / SCO / 精細胞 / Setoli 細胞 / Leydig 細胞
Outline of Final Research Achievements

We investigated the functions of Sertoli cells in severe oligozoospermia and in a patient with obstructive azoospermia as normal spermatogenesis testis. We performed immunohistochemical staining of anti-mullerian hormone (AMH). In immunohistochemical staining of Sertoli cells, there were all positive reactions in the oligozoo group, and one positive reaction in the MESA group in AMH staining. Functional analysis of CK18- and AMH-positive Sertoli cells may facilitate the elucidation of spermatogenesis dysfunction.
Isolation is required to investigate the role of testicular somatic cells, such as Leydig and Sertoli cells, in spermatogenesis.Despite difficulty in testicular isolation of Sertoli cells using DSA lectin and Percoll, Leydig cells could be isolated efficiently.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2017 2016

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (11 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Effect of obesity on sperm retrieval outcome and reproductive hormone levels inJapanese azoospermic men with and without Klinefelter syndrome2017

    • Author(s)
      Iwatsuki Shoichiro, Sasaki Shoichi, Taguchi Kazumi, Mizuno Kentaro, Okada Atsushi, Kubota Yasue, Umemoto Yukihiro, Hayashi Yutaro, Yasui Takahiro
    • Journal Title

      Andrology

      Volume: 5 Issue: 1 Pages: 82-86

    • DOI

      10.1111/andr.12281

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Damege to seminiferous tubules in patients with Sertoli cell-only syndrome progresses with aging.2017

    • Author(s)
      Iwatsuki Shoichiro, Umemoto Yukihiro, Takeda Tomoki, Nozaki Satoshi, Takada Hideki, Kubota Hiroki, Itoh Yasunori, Sasaki Shoichi, Yasui Takahiro
    • Organizer
      第105回日本泌尿器科学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Gene function analysis of Kdm5a, a histone demethylase, in testicular dysfunction.2017

    • Author(s)
      Nishio Hidenori, Mizuno Kentaro, Kato Taiki, Moritoki Yoshinobu, Umemoto Yukihiro, Hayashi Yutaro, Yasui Takahiro
    • Organizer
      第105回日本泌尿器科学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Damage to seminiferous tubules in patients with sertoli cell only syndrome progresses with aging.2017

    • Author(s)
      Iwatsuki Shoichiro, Umemoto Yukihiro, Takeda Tomoki, Nozaki Satoshi, Takada Hideki, Kubota Hideki, Itoh Yasunori, Sasaki Shoichi, Yasui Takahiro
    • Organizer
      American Urological Association Annual Meeting 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Low sample volume cases have high sperm retrieval rates in microdissection testicular sperm extraction.2017

    • Author(s)
      Nozaki Satoshi, Takeda Tomoki, Iwatsuki Shoichiro, Hamakawa Takashi, Taguchi Kazumi, Fujii Yasuhiro, Kubota Hiroki, Kamiya Hiroyuki, Umemoto Yukihiro, Yasui Takahiro
    • Organizer
      American Urological Association Annual Meeting 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 顕微鏡下精巣内精子採取術に対する採精予測因子の検討―Sertoli cell only syndromeとInhibin-Bの関連―2017

    • Author(s)
      梅本 幸裕、岩月 正一郎、佐々木 昌一、武田 知樹、野崎 哲史、窪田 泰江、窪田 裕樹、佐藤 剛、杉浦 真弓、安井 孝周
    • Organizer
      第62回日本生殖医学会学術講演会・総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ホルモン補充療法における精巣内組織変化の検討2016

    • Author(s)
      梅本 幸裕、岩月 正一郎、佐々木 昌一、武田 知樹、野崎 哲史、窪田 裕樹、窪田 泰江、松川 泰、佐藤 剛、杉浦 真弓、安井 孝周
    • Organizer
      第61回日本生殖医学会学術講演会・総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-03
    • Related Report
      2016 Research-status Report
  • [Presentation] クラスター分析を用いたSertoli cell only症候群(SCOS)の新たな分類の試み2016

    • Author(s)
      岩月 正一郎、佐々木 昌一、武田 知樹、西尾 英紀、惠谷 俊紀、神沢 英幸、水野 健太郎、窪田 裕樹、梅本 幸裕、林 祐太郎、安井 孝周
    • Organizer
      日本アンドロロジー学会第35回学術大会
    • Place of Presentation
      前橋テルサ(群馬県前橋市)
    • Year and Date
      2016-06-24
    • Related Report
      2016 Research-status Report
  • [Presentation] ホルモン補充療法における精巣内組織変化の一例2016

    • Author(s)
      梅本 幸裕、佐々木 昌一、武田 知樹、野崎 哲史、岩月 正一郎、窪田 裕樹、松川 泰、出原 麻里、佐藤 剛、杉浦 真弓、郡 健二郎、安井孝周
    • Organizer
      第38回中部生殖医学会学術集会
    • Place of Presentation
      アストプラザ(三重県津市)
    • Year and Date
      2016-06-18
    • Related Report
      2016 Research-status Report
  • [Presentation] Chromosomal anomalies and sperm retrieval rate of patients with nonobstructive azoospermia.2016

    • Author(s)
      Takeda Tomoki, Iwatsuki Shoichiro, T Hamakawa akashi, Kamiya Hiroyuki, Umemoto Yukihiro, Kubota Hiroki, Kubota Yasue, Sasaki Shoichi, Yasui Takahiro
    • Organizer
      American Urological Association Annual Meeting 2016
    • Place of Presentation
      San Diego,USA
    • Year and Date
      2016-05-06
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 造精機能障害時におけるセルトリ細胞の機能変化について2016

    • Author(s)
      梅本幸裕、佐々木昌一、岩月正一郎 ほか
    • Organizer
      第104回 日本泌尿器科学会総会
    • Place of Presentation
      仙台
    • Year and Date
      2016-04-23
    • Related Report
      2015 Research-status Report
  • [Presentation] クラスター分析を用いたSertoli cell only症候群(SCOS)の新たな分類法の開発2016

    • Author(s)
      岩月正一郎、佐々木昌一、梅本幸裕 ほか
    • Organizer
      第104回 日本泌尿器科学会総会
    • Place of Presentation
      仙台
    • Year and Date
      2016-04-23
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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