Elucidation of signal transduction mechanism in the testis and its application to trreatment of male infertility.
| Project/Area Number |
15K10645
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Nagoya City University |
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Principal Investigator |
Umemoto Yukihiro 名古屋市立大学, 大学院医学研究科, 教授 (80381812)
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| Co-Investigator(Kenkyū-buntansha) |
窪田 裕樹 名古屋市立大学, 大学院医学研究科, 研究員 (10347403)
佐々木 昌一 名古屋市立大学, 大学院医学研究科, 研究員 (50225869)
水野 健太郎 名古屋市立大学, 大学院医学研究科, 講師 (70448710)
岩月 正一郎 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (70595397)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 男性不妊症 / Sertoli細胞 / Leydig細胞 / 遺伝子導入 / ＳＣＯ / anti Mullerian hormone / パーコール法 / Ｓｅｒｔｏｌｉ細胞 / SCO / 精細胞 / Setoli 細胞 / Leydig 細胞 |
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| Outline of Final Research Achievements |
We investigated the functions of Sertoli cells in severe oligozoospermia and in a patient with obstructive azoospermia as normal spermatogenesis testis. We performed immunohistochemical staining of anti-mullerian hormone (AMH). In immunohistochemical staining of Sertoli cells, there were all positive reactions in the oligozoo group, and one positive reaction in the MESA group in AMH staining. Functional analysis of CK18- and AMH-positive Sertoli cells may facilitate the elucidation of spermatogenesis dysfunction.
Isolation is required to investigate the role of testicular somatic cells, such as Leydig and Sertoli cells, in spermatogenesis.Despite difficulty in testicular isolation of Sertoli cells using DSA lectin and Percoll, Leydig cells could be isolated efficiently.
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Report
(4 results)
Research Products
(12 results)
