Prolongation of graft survival through peripheral tolerance mechanism via plasmacytoid dendritic cells

• Project/Area Number: 15K10649
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Keio University
• Principal Investigator: Shinoda Kazunobu 慶應義塾大学, 医学部(信濃町), 講師 (60348737)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 末梢性免疫寛容 / pDC / 樹状細胞 / 免疫寛容

Outline of Final Research Achievements

The aim of this study is to investigate the mechanism of spontaneous tolerance in mice kidney transplantation. We employed the tolerance combination (group A: DBA→C57/BL6) and non-tolerance combination (group B: C3H→C57/BL6) and cocultured donor type plasmacytoid DC and recipient type naive T cells. The induction rate of Tregs were significantly higher in group A (10-15%) than in group B (3-5%). Significant prolonged heart allograft survival was observed in group A by administering co-cultured cells containing induced Tregs a week before transplant.