Genomic imprinting in the human placenta

• Project/Area Number: 15K10657
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Tohoku University
• Principal Investigator: Okae Hiroaki 東北大学, 医学系研究科, 助教 (10582695)
• Co-Investigator(Kenkyū-buntansha): 有馬 隆博 東北大学, 医学系研究科, 教授 (80253532)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ゲノムインプリンティング / 胎盤 / 次世代シーケンサー / DNAメチル化 / 栄養膜幹（TS）細胞 / 胎盤幹（TS）細胞

Outline of Final Research Achievements

Genomic imprinting is essential for normal placental development. Here we conducted a genome-wide survey of imprinted genes in the human placenta and identified 44 novel imprinted genes. At least 27 of them were likely to be regulated by DNA methylation. Most of the novel imprinted genes were not imprinted in mouse or mule placenta, suggesting that genomic imprinting in the placenta is highly variable among mammalian species.

Research Products

• [Journal Article] Derivation of Human Trophoblast Stem Cells (2018)
  • Author(s): Okae Hiroaki、Toh Hidehiro、Sato Tetsuya、Hiura Hitoshi、Takahashi Sota、Shirane Kenjiro、Kabayama Yuka、Suyama Mikita、Sasaki Hiroyuki、Arima Takahiro
  • Journal Title: Cell Stem Cell Volume: 22 Issue: 1 Pages: 50-63.e6
  • DOI: 10.1016/j.stem.2017.11.004
  • Peer Reviewed / Open Access
• [Journal Article] Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer (2017)
  • Author(s): Hiura Hitoshi、Hattori Hiromitsu、Kobayashi Norio、Okae Hiroaki、Chiba Hatsune、Miyauchi Naoko、Kitamura Akane、Kikuchi Hiroyuki、Yoshida Hiroaki、Arima Takahiro
  • Journal Title: Clinical Epigenetics Volume: 9 Issue: 1
  • DOI: 10.1186/s13148-017-0379-6
  • Peer Reviewed / Open Access
• [Journal Article] Factors associated with aberrant imprint methylation and oligozoospermia (2017)
  • Author(s): Kobayashi N, Miyauchi N, Tatsuta N, Kitamura A, Okae H, Hiura H, Sato A, Utsunomiya T, Yaegashi N, Nakai K, Arima T
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 42336-42336
  • DOI: 10.1038/srep42336
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genome-Scale Assessment of Age-Related DNA Methylation Changes in Mouse Spermatozoa. (2016)
  • Author(s): Kobayashi N, Okae H, Hiura H, Chiba H, Shirakata Y, Hara K, Tanemura K, Arima T.
  • Journal Title: PLoS One Volume: 11 Issue: 11 Pages: e0167127-e0167127
  • DOI: 10.1371/journal.pone.0167127
  • Peer Reviewed / Open Access
• [Journal Article] Allele-specific methylome and transcriptome analysis reveals widespread imprinting in the human placenta (2016)
  • Author(s): Hamada, H., Okae, H., Toh, H., Chiba, H., Hiura, H., Shirane, K., Sato, T., Suyama, M., Yaegashi, N., Sasaki, H. & Arima
  • Journal Title: The American Journal of Human Genetics Volume: 99 Issue: 5 Pages: 1045-1058
  • DOI: 10.1016/j.ajhg.2016.08.021
  • Peer Reviewed / Open Access
• [Journal Article] DNA methylation dynamics during early human development (2016)
  • Author(s): Okae H, Arima T
  • Journal Title: Journal of Mammalian Ova Research Volume: 33 Issue: 2 Pages: 101-107
  • DOI: 10.1274/jmor.33.101
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] DNA methylation errors in imprinting disorders and male infertility. (2015)
  • Author(s): Kitamura A, Hiura H, Miyauchi N, Okae H, Chiba H, Arima T.
  • Journal Title: Congenit Anom (Kyoto) Volume: 55 Pages: 133-144
• [Presentation] Transposable element expression in placental development (2018)
  • Author(s): Nehemiah S. Alvarez, Kesiuke Kozai, Damayanti Chakraborty, Khursheed Iqbal, Kaela M. Varberg, Pavla Brachova, Hiroaki Okae, Takahiro Arima, Gerald G. Schumann, Kathleen H. Burns and Michael J. Soares
  • Organizer: KEYSTONE SYMPOSIUM 2018
  • Int'l Joint Research
• [Presentation] ヒト胎盤栄養膜幹細胞の樹立とエピゲノム特性 (2017)
  • Author(s): 岡江寛明、有馬隆博
  • Organizer: 第25回日本胎盤学会学術集会
  • Invited
• [Presentation] Derivation of Human Trophoblast Stem Cells from Blastocysts and Villous Cytotrophoblast Cells (2017)
  • Author(s): Arima T, Okae H
  • Organizer: IHEC Science Days & Annual Meeting 2017
• [Presentation] Establishment of human trophoblast stem cells (2017)
  • Author(s): 岡江寛明
  • Organizer: 京大医学部セミナー
  • Invited
• [Presentation] Incomplete reprogramming of germline DNA methylation in the human placenta (2016)
  • Author(s): Arima T, Okae H
  • Organizer: IHEC Science Days & Annual Meeting 2016
  • Place of Presentation: Crowne Plaza Brussels-LePalace（Belgium、Brussels）
  • Year and Date: 2016-09-06
  • Int'l Joint Research
• [Presentation] Transcriptome profiling of purified human trophoblast cells (2016)
  • Author(s): Hamada H, Sato T, Okae H, Yaegashi N, and Arima T.
  • Organizer: 63th Annual Scientific Meeting
  • Place of Presentation: Montreal, QC, Canada
  • Year and Date: 2016-03-16
  • Int'l Joint Research
• [Presentation] ヒト凍結融解胚移植胎盤におけるmicroRNAの網羅的解析 (2016)
  • Author(s): 樋浦仁、服部裕充、岡江寛明、千葉初音、宮内尚子、北村茜、菊地裕幸、吉田秋仁、有馬隆博
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
• [Presentation] 繁殖期におけるマウス精子DNAメチル化様式の加齢性変化 (2016)
  • Author(s): 小林記緒、岡江寛明、樋浦仁、千葉初音、白形芳樹、原健士郎、種村健太郎、有馬隆博
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
• [Presentation] 分化多能性を持つヒト胎盤栄養膜幹細胞の樹立 (2016)
  • Author(s): 岡江寛明、有馬隆博
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Invited
• [Presentation] ウシ卵胞内卵子の発生能を制御する卵丘細胞シグナルネットワーク (2016)
  • Author(s): 杉村智史、岡江寛明、有馬隆博
  • Organizer: 第109回日本繁殖生物学会大会
  • Place of Presentation: 麻布大学（神奈川県相模原市）
• [Presentation] ヒト胚発生におけるエピジェネティクスの変調 (2015)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、千葉初音、宮内尚子
  • Organizer: 第33回受精着床学会
  • Place of Presentation: TFTホール（東京都江東区）
  • Year and Date: 2015-11-26
  • Invited
• [Presentation] ヒト胎盤におけるインプリント型X染色体不活性化 (2015)
  • Author(s): 濱田裕貴、岡江寛明、八重樫伸生、有馬隆博
  • Organizer: 第23回日本胎盤学会学術集会
  • Place of Presentation: JA共済ビル（東京都千代田区）
  • Year and Date: 2015-11-05
• [Presentation] DNA methylation dynamics and regulation of genomic imprinting during human development (2015)
  • Author(s): Okae H, Arima T.
  • Organizer: EMBO Workshop
  • Place of Presentation: Gottingen, Germany
  • Year and Date: 2015-05-06
  • Int'l Joint Research
• [Book] 実験医学 (2018)
  • Author(s): 岡江寛明、有馬隆博
  • Total Pages: 1430
  • Publisher: 羊土社
• [Book] 新着論文レビュー (2018)
  • Author(s): 岡江寛明、有馬隆博
  • Publisher: ライフサイエンス
• [Book] Epigenetics in Human Disease, Second Edition (2018)
  • Author(s): Hiura H, Hattori H, Kobayashi N, Takahashi S, Okae H, Arima T
  • Publisher: Elsevier
• [Book] Handbook of Nutrition, Diet, and Epigenetics (2017)
  • Author(s): Miyauchi N, Kitamura A, Hiura H, Okae H, Kobayashi N, Hattori H, Takahashi S, Arima T
  • Publisher: Springer International Publishing
• [Book] 生殖補助医療(ART) -胚培養の理論と実際- (2017)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、佐藤晶子
  • Total Pages: 352
  • Publisher: 近代出版
• [Book] Pharma Medica (2016)
  • Author(s): 有馬隆博、宮内尚子、北村茜、樋浦仁、岡江寛明、千葉初音
  • Total Pages: 144
  • Publisher: メディカルレビュー社