Maintenance of germ cell quality via mitochondrial quality control

• Project/Area Number: 15K10663
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: University of Fukui
• Principal Investigator: ORISAKA Makoto 福井大学, 学術研究院医学系部門(附属病院部), 講師 (80324143)
• Co-Investigator(Renkei-kenkyūsha): NAKAMURA Yasuyuki 独立行政法人国立がん研究センター, 腫瘍生物学分野, 主任研究員 (90569063)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 精子 / 卵子 / ミトコンドリア / 酸化ストレス / 加齢 / ミトコンドリア品質管理 / Mieap

Outline of Final Research Achievements

The present study demonstrates that mitochondrial quality control system plays an important role to maintain sperm quality. The failure of the mitochondrial quality control system with aging seems to induce oxidative stress in sperm, resulting poor sperm quality and poor embryo quality. On the contrary, the mitochondrial quality control system is not involved in poor oocyte quality with aging.

Research Products

• [Int'l Joint Research] オタワ大学(カナダ)
• [Journal Article] Luteinizing Hormone Facilitates Antral Follicular Maturation and Survival via Thecal Paracrine Signaling in Cattle. (2018)
  • Author(s): Hattori K, Orisaka M, Fukuda S, Tajima K, Yamazaki Y, Mizutani T, Yoshida Y.
  • Journal Title: Endocrinology Volume: 印刷中 Issue: 6 Pages: 2337-2347
  • DOI: 10.1210/en.2018-00123
  • Peer Reviewed
• [Journal Article] Saikosaponin-d, a calcium mobilizing agent, sensitizes chemoresistant ovarian cancer cells to cisplatin-induced apoptosis by facilitating mitochondrial fission and G2/M arrest. (2017)
  • Author(s): H.Tsuyoshi, VKW.Wong, Y.Han, M.Orisaka, Y.Yoshida, BK.Tsang
  • Journal Title: Oncotarget Volume: 8（59） Issue: 59 Pages: 99825-99840
  • DOI: 10.18632/oncotarget.21076
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 卵胞発育と卵子・顆粒膜細胞・莢膜細胞の相互応答 (2016)
  • Author(s): 折坂 誠，白藤 文，玉村 千代
  • Journal Title: 臨床婦人科産科 Volume: 70(12) Pages: 1104-8
  • Peer Reviewed
• [Journal Article] 【2.不妊症の検査・診断】≪女性因子≫ 卵胞発育モニタリングの実施法 (2016)
  • Author(s): 折坂 誠，福田 真，吉田 好雄
  • Journal Title: 臨床婦人科産科 Volume: 70(4) Pages: 62-5
  • Peer Reviewed
• [Journal Article] 【2.不妊症の検査・診断】 卵胞発育モニタリングで卵胞が見えない場合、どのように対処すればよいですか？ (2016)
  • Author(s): 折坂 誠，福田 真，吉田 好雄
  • Journal Title: 臨床婦人科産科 Volume: 70(4) Pages: 124-6
  • Peer Reviewed
• [Journal Article] Amniotic Fluid Embolism Triggered By Hypertensive Crisis Due to Undiagnosed Pheochromocytoma in a Pregnant Subject With Neurofibromatosis Type 1． (2015)
  • Author(s): N.Takahashi, K.Nishijima, M.Orisaka, H.Tsuyoshi, T.Kurokawa, Kana Kato, A.Shirafuji, K.Arakawa, K.Hisazaki, H.Tada, Y.Yoshida
  • Journal Title: AACE Clinical Case Rep. Volume: 1（3） Issue: 3 Pages: e178-e181
  • DOI: 10.4158/ep14108.cr
  • Peer Reviewed
• [Journal Article] Ovarian mucinous adenocarcinoma with functioning stroma in postmenopausal women: aromatase and SF-1 expressions. (2015)
  • Author(s): Y.Hattori, S.Yamada, M.Yamamoto, M.Orisaka, T.Mizutani, Y.Yoshida
  • Journal Title: J Ovarian Res. Volume: 8 Issue: 1 Pages: 73-73
  • DOI: 10.1186/s13048-015-0202-y
  • Peer Reviewed / Open Access
• [Journal Article] Mitochondrial dynamics regulating chemoresistance in gynecological cancers． (2015)
  • Author(s): B.Kong, H.Tsuyoshi, M.Orisaka, DB.Shieh, Y.Yoshida, BK.Tsang
  • Journal Title: Ann N Y Acad Sci. Volume: 1350 Issue: 1 Pages: 1-16
  • DOI: 10.1111/nyas.12883
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Mitochondria-eating protein is essential for sperm function, but not oocyte quality, in in vitro fertilization (2018)
  • Author(s): Orisaka M, Yoshida Y.
  • Organizer: 第70回日本産科婦人科学会
• [Presentation] Oocyte-granulosa-theca cell interactions during ovarian follicle growth.（Inernational Symposium） (2017)
  • Author(s): M.Orisaka
  • Organizer: The 6th International Symposium of Taiwan Association of Obstetrics and Gynecology.
  • Place of Presentation: Taipei Grand Hotel（Taipei、Taiwan）
  • Year and Date: 2017-03-18
  • Int'l Joint Research / Invited
• [Presentation] LH～莢膜細胞系が卵胞選択を主導する可能性 (2017)
  • Author(s): 服部 克成，折坂 誠，福田 真，吉田 好雄
  • Organizer: 第21回日本生殖内分泌学会学術集会
  • Place of Presentation: 千里ライフサイエンスセンター（大阪府豊中市）
• [Presentation] Oocyte-granulosa-theca cell interactions during early follicular growth. (2016)
  • Author(s): M.Orisaka
  • Organizer: The 8th Japan-Korea ART Conference.
  • Place of Presentation: ワークピア横浜（神奈川県横浜市）
  • Int'l Joint Research
• [Presentation] Oocyue-Granulosa-Theca Cell Interactions during Early Follicular Growth (2015)
  • Author(s): M.Orisaka
  • Organizer: IT’s 4th Annual World Congress of Diabetes BIT’s 5th Annual World Congress of Endobolism
  • Place of Presentation: 高雄（台湾）
  • Year and Date: 2015-11-25
  • Invited
• [Presentation] GnRHアゴニストが胎盤ポリープの血流減少に有効だった症例 (2015)
  • Author(s): 折坂 誠
  • Organizer: 中日本産婦人科セミナー
  • Place of Presentation: 富山市
  • Year and Date: 2015-07-04
• [Presentation] Protective effect of dienogest on chemotherapy-induced reduced fertility in female rats (2015)
  • Author(s): M.Orisaka
  • Organizer: IFFS/JSRM International Meeting 2015
  • Place of Presentation: 横浜市
  • Year and Date: 2015-04-26
  • Invited
• [Presentation] 卵胞細胞における卵子～顆粒膜細胞～莢膜細胞間のクロストーク (2015)
  • Author(s): 折坂 誠
  • Organizer: 第67回日本産科婦人科学会学術講演会（シンポジウム）
  • Place of Presentation: 横浜市
  • Year and Date: 2015-04-09
• [Book] 知りたい最新情報がすぐわかる！不妊・不育症診療パーフェクトガイド (2016)
  • Author(s): 折坂 誠，福田 真，吉田 好雄，他
  • Total Pages: 390
  • Publisher: 医学書院