In utero Immune tolerance induction for Severe Congenital skin diseases

• Project/Area Number: 15K10669
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Osaka University
• Principal Investigator: ENDO MASAYUKI 大阪大学, 医学系研究科, 講師 (30644794)
• Co-Investigator(Kenkyū-buntansha): 玉井 克人 大阪大学, 医学系研究科, 寄附講座教授 (20236730) ; 冨松 拓治 大阪大学, 医学(系)研究科(研究院), 講師 (30346209) ; 味村 和哉 大阪大学, 医学(系)研究科(研究院), 助教 (50437422)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 胎児治療 / 免疫寛容 / 幹細胞 / 移植 / マイクロキメリズム / 産科学 / 間葉系幹細胞 / 母体胎児間マイクロキメリズム / 絨毛採取 / 超音波 / 幹細胞移植 / 血液幹細胞

Outline of Final Research Achievements

Induction of immunological reactions is a major problem in replacement therapies for inherited protein deficiencies. Tolerance created in utero can facilitate postnatal treatment. In this study, we show that cell transplantation into the chorionic villi under ultrasound guidance in the earliest gestation can induce immune tolerance towards a foreign protein within rodent models.

Research Products

• [Journal Article] Treatment and repair of uterine scar dehiscence during cesarean section (2017)
  • Author(s): Sawada, M. Matsuzaki, S. Nakae, R. Iwamiya, T. Kakigano, A. Kumasawa, K. Ueda, Y. Endo, M. Kimura, T.
  • Journal Title: Clin Case Rep Volume: 5 Issue: 2 Pages: 145-149
  • DOI: 10.1002/ccr3.766
  • Peer Reviewed / Open Access
• [Journal Article] Successful anticoagulant therapy for disseminated intravascular coagulation during conservative management of placenta percreta: a case report and literature review (2017)
  • Author(s): Matsuzaki, S. Yoshino, K. Endo, M. Tomimatsu, T. Takiuchi, T. Mimura, K. Kumasawa, K. Ueda, Y. Kimura, T.
  • Journal Title: BMC Pregnancy Childbirth Volume: 17 Issue: 1 Pages: 443-443
  • DOI: 10.1186/s12884-017-1634-8
  • Peer Reviewed / Open Access
• [Journal Article] Vasa previa with an intact amniotic membrane (2017)
  • Author(s): Matsuzaki, S. Endo, M. Kimura, T.
  • Journal Title: Am J Obstet Gynecol. Volume: 216 Issue: 6 Pages: 616.e1-616.e2
  • DOI: 10.1016/j.ajog.2017.03.003
  • Peer Reviewed
• [Journal Article] Investigation of perioperative complications associated with uterine compression sutures (2017)
  • Author(s): Suzuki, Y. Matsuzaki, S. Mimura, K. Kumasawa, K. Tomimatsu, T. Endo, M. Kimura, T.
  • Journal Title: Int J Gynaecol Obstet Volume: 139 Issue: 1 Pages: 28-33
  • DOI: 10.1002/ijgo.12249
  • Peer Reviewed
• [Journal Article] Pregnancy outcomes in women with different doses of corticosteroid supplementation during labor and delivery (2017)
  • Author(s): Owa, T. Mimura, K. Kakigano, A. Matsuzaki, S. Kumasawa, K. Endo, M. Tomimatsu, T. Kimura, T.
  • Journal Title: J Obstet Gynaecol Res Volume: 43 Issue: 7 Pages: 1132-1138
  • DOI: 10.1111/jog.13336
  • Peer Reviewed / Open Access
• [Journal Article] A case of acute sheehan’s syndrome and literature review: a rare but life-threatening complication of postpartum hemorrhage (2017)
  • Author(s): Matsuzaki, S. Endo, M. Ueda, Y. Mimura, K. Kakigano, A. Egawa-Takata, T. Kumasawa, K. Yoshino, K. Kimura, T.
  • Journal Title: BMC Pregnancy Childbirth Volume: 17 Issue: 1 Pages: 188-188
  • DOI: 10.1186/s12884-017-1380-y
  • Peer Reviewed / Open Access
• [Journal Article] 胎児遺伝子治療 (2017)
  • Author(s): 遠藤 誠之
  • Journal Title: 周産期医学 Volume: 47 Pages: 571-576
  • Peer Reviewed
• [Journal Article] 胎児期幹細胞移植の現状と将来 (2017)
  • Author(s): 遠藤 誠之
  • Journal Title: 医学のあゆみ Volume: 261 Pages: 1229-1234
  • Peer Reviewed
• [Journal Article] Physician-initiated first-in-human clinical study using a novel angiogenic peptide, AG30/5C, for patients with severe limb ulcers (2017)
  • Author(s): Nakagami Hironori、Yamaoka Toshifumi、Hayashi Misa、Tanemura Atsushi、Takeya Yasushi、Kurinami Hitomi、Sugimoto Ken、Nakamura Ayumi、Tomono Kazunori、Tamai Katsuto、Katayama Ichiro、Rakugi Hiromi、Kaneda Yasufumi
  • Journal Title: Geriatr Gerontol Int. Volume: 17 Issue: 11 Pages: 2150-2156
  • DOI: 10.1111/ggi.13051
  • Peer Reviewed / Open Access
• [Journal Article] Pathophysiology of preeclampsia: an angiogenic imbalance and long-lasting systemic vascular dysfunction. (2016)
  • Author(s): Tomimatsu T, Mimura K, Endo M, Kumasawa K, Kimura T.
  • Journal Title: Hypertens Res Volume: 40(4) Issue: 4 Pages: 305-310
  • DOI: 10.1038/hr.2016.152
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Expression levels of ABCG2 on cord red blood cells and study of fetal anemia associated with anti-Jr(a). (2016)
  • Author(s): Fujita S, Kashiwagi H, Tomimatsu T, Ito S, Mimura K, Kanagawa T, Endo M, Miyoshi T, Okamura Y, Tani Y, Tomiyama Y, Kimura T.
  • Journal Title: Transfusion. Volume: 56 Issue: 5 Pages: 1171-1181
  • DOI: 10.1111/trf.13515
  • Peer Reviewed / Open Access
• [Journal Article] Cervical varix complicated by placenta previa: A case report and literature review. (2016)
  • Author(s): Tanaka M, Matsuzaki S, Kumasawa K, Suzuki Y, Endo M, Kimura T.
  • Journal Title: J Obstet Gynaecol Res. Volume: 42 Issue: 7 Pages: 883-889
  • DOI: 10.1111/jog.12969
  • Peer Reviewed / Open Access
• [Journal Article] Placenta accreta following hysteroscopic myomectomy. (2016)
  • Author(s): Tanaka M, Matsuzaki S, Matsuzaki S, Kakigano A, Kumasawa K, Ueda Y, Endo M, Kimura T.
  • Journal Title: Clin Case Rep Volume: 4(6) Issue: 6 Pages: 541-544
  • DOI: 10.1002/ccr3.562
  • Peer Reviewed / Open Access
• [Journal Article] Successful conservative management of placenta percreta: Investigation by serial magnetic resonance imaging of the clinical course and a literature review. (2016)
  • Author(s): Sawada M, Matsuzaki S, Mimura K, Kumasawa K, Endo M, Kimura T.
  • Journal Title: J Obstet Gynaecol Res. Volume: 42 Issue: 12 Pages: 1858-1863
  • DOI: 10.1111/jog.13121
  • Peer Reviewed / Open Access
• [Journal Article] Pulmonary transcriptome analysis in the surgically induced rabbit model of diaphragmatic hernia treated with fetal tracheal occlusion (2016)
  • Author(s): Engels, A. C. Brady, P. D. Kammoun, M. Finalet Ferreiro, J. DeKoninck, P. Endo, M. Toelen, J. Vermeesch, J. R. Deprest, J.
  • Journal Title: Dis Model Mech. Volume: 9 Pages: 221-228
  • DOI: 10.1242/dmm.021626
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Immune tolerance induction using fetal directed placental injection in rodent models: a murine model. (2015)
  • Author(s): Takahashi, K. Endo, M. Miyoshi, T. Tsuritani, M. Shimazu, Y. Hosoda, H. Saga, K. Tamai, K. Flake, AW. Yoshimatsu, J. Kimura, T.
  • Journal Title: PLoS One. Volume: 10(4) Issue: 4 Pages: e0123712-e0123712
  • DOI: 10.1371/journal.pone.0123712
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] High-frequency micro-ultrasound: a novel method to assess external urethral sphincter function in rats following simulated birth injury (2015)
  • Author(s): Hakim, L. Endo, M. Feola, A. Soebadi, D. M. Deprest, J. De Ridder, D. Albersen, M. Van der Aa, F.
  • Journal Title: Neurourology and Urodynamics Volume: 34 Issue: 3 Pages: 264-269
  • DOI: 10.1002/nau.22555
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Thrombocytopenia associated with Mycoplasma pneumonia during pregnancy: Case presentation and approach for differential diagnosis (2015)
  • Author(s): Nishikawa, A. Mimura, K. Kanagawa, T. Maeda, T. Tomimatsu, T. Kimura, T.
  • Journal Title: Journal of Obstetrics and Gynaecology Research Volume: 41 Issue: 8 Pages: 1273-1277
  • DOI: 10.1111/jog.12713
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Pregnancy management for a patient with graft occlusion after right iliac artery bypass surgery (2015)
  • Author(s): Nakae, R. Matsuzaki, S. Egawa-Takata, T. Mimura, K. Kanagawa, T. Kimura, T.
  • Journal Title: Journal of Obstetrics and Gynaecology Research Volume: 41 Issue: 6 Pages: 979-984
  • DOI: 10.1111/jog.12636
  • Peer Reviewed / Open Access
• [Journal Article] Drug repositioning for preeclampsia therapeutics by in vitro screening: phosphodiesterase-5 inhibitor vardenafil restores endothelial dysfunction via induction of placental growth factor (2015)
  • Author(s): Kakigano, A. Tomimatsu, T. Mimura, K. Kanayama, T. Fujita, S. Minato, K. Kumasawa, K. Taniguchi, Y. Kanagawa, T. Endo, M. Ishihara, T. Namba, T. Mizushima, T. Kimura, T.
  • Journal Title: Reproductive Sciences Volume: 10 Issue: 10 Pages: 1272-80
  • DOI: 10.1177/1933719115574340
  • Peer Reviewed
• [Presentation] Intra-Chorionic Villi Injection At Early Gestational Stage Can Induce Immue Tolerance Towards Allogeneic Cells In A Murine Model. (2017)
  • Author(s): Endo M.
  • Organizer: 3rd Meeting of the International Fetal Immunology and Trasplantations Society
  • Place of Presentation: Philadelphia(U.S.A)
  • Year and Date: 2017-03-23
  • Int'l Joint Research
• [Presentation] Intra-Chorionic Villi Injection at Early Gestational Stage Can Induce Immune Tolerance Towards Allogeneic Cells In A Murine Model. (2017)
  • Author(s): Endo M, Shimazu Y, Okada A.
  • Organizer: 3rd Meeting of the International Fetal Immunology and Transplantations Society
  • Int'l Joint Research
• [Presentation] A 9-years Experience of Induced trimester abortion is a single tertiary refferal center. (2017)
  • Author(s): Endo M, Mimura K, Tomimatsu T, Matsuzaki S, Kumasawa K, Matsumoto Y, Kinose Y, Iwamiya T, Kimura T.
  • Organizer: 第69回日本産科婦人科学会学術講演会
• [Presentation] 胎児共存奇胎とその鑑別を要した6症例の検討 (2017)
  • Author(s): 味村 和哉, 遠藤 誠之, 柿ヶ野 藍子, 木村 正.
  • Organizer: 第53回日本周産期・新生児医学会学術集会
• [Presentation] 当院における妊娠中期中絶症例について (2017)
  • Author(s): 遠藤 誠之, 松崎 慎也, 熊澤 惠一, 味村 和哉, 瀧内 剛, 木村 正.
  • Organizer: 第53回日本周産期・新生児医学会学術集会
• [Presentation] 胎児治療最前線 (2016)
  • Author(s): 遠藤 誠之
  • Organizer: 日本臨床麻酔学会 第36回大会
  • Place of Presentation: 高知
  • Year and Date: 2016-11-03
  • Invited
• [Presentation] Prenatal Diagnosis of Airway Obstruction Improves Perinatal Outcomes. (2016)
  • Author(s): Endo M
  • Organizer: 12th Asia Pacific Congress in Maternal Fetal Medicine
  • Place of Presentation: Malaysia(Penang)
  • Year and Date: 2016-10-06
  • Int'l Joint Research
• [Presentation] In utero stem cell and gene therapy (2016)
  • Author(s): Endo M.
  • Organizer: The 60th Anniversary Celebration Educational Seminar
  • Place of Presentation: ウランバートル（モンゴル）
  • Year and Date: 2016-09-07
  • Invited
• [Presentation] Immune tolerance induction using fetal directed placental injection in rodent models. (2016)
  • Author(s): Endo M.
  • Organizer: 12th Congress of International Academy of Perinatal Medicine
  • Place of Presentation: Tirana(Albania)
  • Year and Date: 2016-05-27
  • Int'l Joint Research
• [Presentation] In utero stem cell therapy for Osteogenesis Imperfecta : a clinical case report (2016)
  • Author(s): Endo M, Takahashi K, Ito Y, Kanagawa T, Fukui O, Mimura K, Kakigano A, Shimazu Y, Kajimoto E, Tomimatsu T, Kimura T.
  • Organizer: 第68回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2016-04-21
• [Presentation] Shunting Instruments - which is best (2016)
  • Author(s): Endo M.
  • Organizer: Asia Pacific Conference on Fetal Therapy 2016
  • Place of Presentation: Singapore(National University of Singapore)
  • Year and Date: 2016-04-16
  • Int'l Joint Research
• [Presentation] USG and MRI of pelvic floor during pregnancy and postpartum (2015)
  • Author(s): Endo, M.
  • Organizer: 11th Asia Pacific Congress in Maternal Fetal Medicine
  • Place of Presentation: Taiwan, China
  • Year and Date: 2015-11-27
  • Int'l Joint Research / Invited
• [Book] 新生児先天性横隔膜ヘルニア(CDH)診療ガイドライン (2016)
  • Author(s): 遠藤 誠之、臼井 規朗、左合 治彦 他新生児先天性横隔膜ヘルニア研究グループ
  • Total Pages: 114
  • Publisher: メジカルビュー社
• [Book] 小児外科 (2016)
  • Author(s): 遠藤 誠之、和田 誠司、左合 治彦、木村 正 他
  • Total Pages: 120
  • Publisher: 東京医学社