Redox regulation of estrogen and miRNA-222 in adipocytes

Research Project

Project/Area Number	15K10700
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Obstetrics and gynecology
Research Institution	Yamagata University
Principal Investigator	Takahashi Kazuhiro 山形大学, 医学部, 非常勤講師 (20292427)
Co-Investigator(Kenkyū-buntansha)	山谷日鶴山形大学, 医学部, 助教 (40550637)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	エストロゲン / 閉経 / 内臓脂肪 / 酸化ストレス / ROS
Outline of Final Research Achievements	The size of visceral adipocytes in postmenopausal women was significantly larger than that in premenopausal women (P < 0.05). The lipid peroxidation levels of visceral fat in postmenopal women were also significantly higher than that in premenopausal women (P < 0.05). We found that oxidative stress in visceral fat is higher in postmenopausal women. Estradiol (E2) significantly reduced the intracellular ROS levels that were induced by H2O2 in 3T3-L1 adipocytes (P < 0.01). We determined that E2 significantly increased the expression of nuclear factor erythroid 2-related factor 2 (NRF2)-dependent antioxidant genes, heme oxygenase-1 (HO-1), NAD(P)H:quinone oxidoreductase 1 (NQO-1), and the glutamate-cysteine ligase modifier subunit (GCL) genes in 3T3-L1 adipocytes (P < 0.01). Hence, estrogen may act as an antioxidant in the adipose tissues of premenopausal women.