Clarification of molecular biological feature of Mullerian endometrioid adenocarcinoma and establishment of differentiation of its origin

• Project/Area Number: 15K10707
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Niigata University
• Principal Investigator: YAMAGUCHI Masayuki 新潟大学, 医歯学総合病院, 講師 (20529771)
• Co-Investigator(Kenkyū-buntansha): 吉原 弘祐 新潟大学, 医歯学系, 助教 (40547535) ; 関根 正幸 新潟大学, 医歯学総合病院, 助教 (70345502) ; 榎本 隆之 新潟大学, 医歯学系, 教授 (90283754)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ミュラー管由来類内膜腺癌 / 類内膜癌関連遺伝子 / Endometrioid carcinoma / Transcriptome / translational research / cancer / mutation

Outline of Final Research Achievements

Microarray analyses were performed between uterine corpus endometrioid adenocarcinoma group (n=73), uterine corpus serous adenocarcinoma group (n=12), ovarian endometorioid adenocarcinoma group (n=20), and ovarian serous adenocarcinoma group (n=243). Consequently, 135 genes associated with ovarian endometorioid adenocarcinoma and 102 genes associated with uterine corpus endometrioid adenocarcinoma were extracted. We compared gene expression profiling of ovarian endometrioid adenocarcinoma with that of uterine corpus endometrioid adenocarcinoma and validated reproducibility using TCGA database. Finally, difference in degree of PI3K-AKT pathway activation between ovarian endometrioid adenocarcinoma and uterine corpus endometrioid adenocarcinoma was revealed with single sample gene set enrichmint analysis.

Research Products

• [Journal Article] Malignancy during pregnancy in Japan: an exceptional opportunity for early diagnosis. (2018)
  • Author(s): Sekine M, Kobayashi Y, Tabata T, Sudo T, Nishimura R, Matsuo K, Grubbs BH, Enomoto T, Ikeda T.
  • Journal Title: BMC Pregnancy Childbirth. Volume: 18(1) Issue: 1 Pages: 50-50
  • DOI: 10.1186/s12884-018-1678-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Novel therapeutic strategy for cervical cancer harboring FGFR3-TACC3 fusions (2018)
  • Author(s): Tamura R, Yoshihara K, Saito T, Ishimura R, Martinez-Ledesma JE, Xin H, Ishiguro T, Mori Y, Yamawaki K, Suda K, Sato S, Itamochi H, Motoyama T, Aoki Y, Okuda S, Casingal CR, Nakaoka H, Inoue I, Verhaak RGW, Komatsu M, Enomoto T.
  • Journal Title: Oncogenesis Volume: 7 Issue: 1 Pages: 4-4
  • DOI: 10.1038/s41389-017-0018-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sox2-dependent inhibition of p21 is associated with poor prognosis of endometrial cancer. (2017)
  • Author(s): Yamawaki K, Ishiguro T, Mori Y, Yoshihara K, Suda K, Tamura R, Yamaguchi M, Sekine M, Kashima K, Higuchi M, Fujii M, Okamoto K, Enomoto T
  • Journal Title: Cancer Sci. Volume: 108 Issue: 4 Pages: 632-640
  • DOI: 10.1111/cas.13196
  • Peer Reviewed / Open Access