| Project/Area Number |
15K10713
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Aichi Cancer Center Research Institute |
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Principal Investigator |
Mika Mizuno 愛知県がんセンター(研究所), 腫瘍免疫学部, 研究員 (50588837)
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| Co-Investigator(Kenkyū-buntansha) |
柴田 清住 藤田保健衛生大学, 医学部, 教授 (90335026)
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| Co-Investigator(Renkei-kenkyūsha) |
KAJIYAMA Hiroaki 名古屋大学, 医学系研究科, 准教授 (00345886)
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| Research Collaborator |
TESHIGAWARA Toshiya
ISHII Takuya
ISHIZUKA Masahiro
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 5-aminolevulinic acid / Photodynamic therapy / 卵巣がん / 難治性癌 / photodynamic therapy / 5-アミノレブリン酸 / 癌幹細胞 / 卵巣癌 / ５ALA / 光線力学療法 |
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| Outline of Final Research Achievements |
Photodynamic therapy (PDT) is known as a minimally invasive treatment for cancer. 5-Aminolevulinic acid (ALA) is a precursor of the photosensitizing agent protoporphyrin IX (PpIX). We evaluated the efficacy of ALA-PDT and investigated relevant factors of enhancement of effect on various ovarian cancer cells in vitro and in vivo.
We confirmed the effect of ALA-PDT using various ovarian cancer cell lines. Especially,we found that three cell lines of clear cell carcinoma, which is chemoresistance tumor,were also highly sensitive to ALA-PDT.The cell lines sensitive to ALA-PDT expressed PEPT1 (an ALA uptake transporter) adn the cell line resistant to ALA-PDT expressed ABCG2 (a PpIX export transporter). In the resistant cell line, a combination treatment with both ALA and an ABCG2 inhibitor resulted in the promotion of cytotoxic sensitivity in vitro.
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