| Project/Area Number |
15K10730
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Keio University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
赤羽 智子 慶應義塾大学, 医学部(信濃町), 特任助教 (40398699)
平沢 晃 慶應義塾大学, 医学部(信濃町), 講師 (90296658)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Keywords | 薬剤感受性 / 卵巣癌 / 初代培養 / 薬剤感受性試験 / ドラッグリポジショニング / precision medicine |
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| Outline of Final Research Achievements |
The following results were raised within this research project.
①We successfully established a new cell line from malignant mesothelioma ascites. This cell line is a valuable biological resource to study the mechanisms underlying carcinogenesis in peritoneal mesothelioma and for drug sensitivity tests (DSRT).②DSRT were conducted in two dimensional and three dimensional cultures using established cell lines. The pemetrexed (PEM) is used for standard therapy of malignant mesothelioma. PEM was drug susceptibility in two dimensional culture by DSRT, but there was no drug effect in three dimensional culture. Those results were consistent with the clinical course and it was possible to conduct a DSRT that mimics the in vivo environment.③Cell cultured from ovarian carcinoma tumor tissue fragments formed spheroids and succeeded in creating a ductal structure similar to the in vivo structure. We succeeded in forming spheroids derived from ovarian cancer tumor tissue.
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