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GPR30 signaling regulates Epithelial-Mesenchymal Transition in ovarian cancer

Research Project

Project/Area Number 15K10738
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionOsaka Medical College

Principal Investigator

Fujiwara Satoe  大阪医科大学, 医学部, 講師 (90707960)

Co-Investigator(Kenkyū-buntansha) 林 正美  大阪医科大学, 医学部, 講師 (00551748)
大道 正英  大阪医科大学, 医学部, 教授 (10283764)
田中 良道  大阪医科大学, 医学部, 講師 (10625502)
金村 昌徳  大阪医科大学, 医学部, 非常勤講師 (40298782)
恒遠 啓示  大阪医科大学, 医学部, 講師 (70388255)
田辺 晃子  大阪医科大学, 医学部, 非常勤講師 (70454543)
佐々木 浩  大阪医科大学, 医学部, 助教 (80432491)
寺井 義人  大阪医科大学, 医学部, 准教授 (90278531)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords卵巣癌 / GPR30 / EMT / EMT現象
Outline of Final Research Achievements

The effect of GPR30 on EMT were evaluated in ovarian cancer cell line, A2780, which were cultured both in two-dimensional (2D) culture and three-dimensional (3D) culture model. GPR30 agonist, G1, was used to confirm the regulatory effects of GPR30 on the change of phenotypic modulation and EMT markers expression.
In 3D culture, the stimulation of GPR30 leads the floating and sphere formation in A2780. G1-induced EMT was observed with related regulation of EMT markers expression at both mRNA and protein level. G1 induced the decrease of E-cadherin level and the increase of Snail and Vimentin in RT-PCR and Western blotting. Knockdown of GPR30, using siRNA, blocked G1-induced EMT. So, GPR30 might be an important molecule related to metastasis process in ovarian cancer.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (1 results)

All 2018

All Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] GPR30 signaling regulates Epithelial-Mesenchymal Transition in ovarian cancer2018

    • Author(s)
      藤原聡枝 田中良道 田中智人 恒遠啓示 佐々木浩 寺井義人 大道正英
    • Organizer
      International Gynecologic Cancer Society 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2019-03-29  

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