Establishment of quantitative methods to evaluate mitochondrial function in the inner ear
| Project/Area Number |
15K10743
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Research Collaborator |
KINOSHITA MAKOTO
KAMOGASHIRA TERU
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 内耳 / ミトコンドリア / 加齢 / 有毛細胞 / 蝸牛 / 電子伝達系 / ミトコンドリア膜電位 / 酸化ストレス |
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| Outline of Final Research Achievements |
We established quantitative method to evaluate mitochondrial membrane potential by using JC-1 fluorescence dye in HEI-OC1 auditory cell line and cochlear explants. We also established quantitative method to evaluate mitochondrial respiratory function by using an extracellular flux analyzer in HEI-OC1 auditory cell line and cochlear explants.
We then evaluated mitochondrial function of a senescence model of HEI-OC1 cells induced by H2O2 exposure, by these established quantitative methods.
We revealed that relative value of oxygen consumption rate on FCCP injection was dose dependently decreased in the H2O2-exposed cells. This suggests damage of the mitochondrial respiratory capacity in the senescence model.We also revealed that JC-1 red/green ratio was decreased in a dose-dependent manner in the H2O2-exposed cells. This suggests decreased mitochondrial membrane potential in the senescence model.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Denervation of nasal mucosa induced by posterior nasal neurectomy suppresses nasal secretion, not hypersensitivity, in an allergic rhinitis rat model.2016
Author(s)
Nishijima H, Kondo K, Toma-Hirano M, Iwasaki S, Kikuta S, Fujimoto C, Ueha R, Kagoya R, Yamasoba T
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Journal Title
Lab Invest
Volume: 96
Issue: 9
Pages: 981-993
DOI
Related Report
Peer Reviewed
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[Journal Article] Association of the upregulated expression of focal adhesion kinase with poor prognosis and tumor dissemination in hypopharyngeal cancer.2016
Author(s)
Omura G, Ando M, Saito Y, Kobayashi K, Yoshida M, Ebihara Y, Kanaya K, Fujimoto C, Sakamoto T, Kondo K, Asakage T, Yamasoba T
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Journal Title
Head Neck.
Volume: Published on line
Issue: 8
Pages: 1164-1169
DOI
Related Report
Peer Reviewed
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[Journal Article] Maxillary carcinosarcoma: Identification of a novel MET mutation in both carcinomatous and sarcomatous components through next generation sequencing2015
Author(s)
Mizuo Ando, Yuki Saito, Teppei Morikawa, Go Omura, Kenya Kobayashi, Ken Akashi, Masafumi Yoshida, Yasuhiro Ebihara, Chisato Fujimoto, Masashi Fukayama, Tatsuya Yamasoba, Takahiro Asakage
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Journal Title
Head and Neck
Volume: 未定
Issue: 12
DOI
Related Report
Peer Reviewed
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