Control of stria vascularis function and changes of auditory and vestibular function induced by optogenetic stimulation

• Project/Area Number: 15K10761
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Kyorin University
• Principal Investigator: Masuda Masatsugu 杏林大学, 医学部, 講師 (20317225)
• Research Collaborator: TANAKA Kenji F 慶應義塾大学, 医学部, 准教授 ; WATABE Takahisa 慶應義塾大学, 医学部, 助教
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: オプトジェネティクス / 蝸牛 / 血管条 / 聴力 / 変動性難聴 / 外側壁 / イオンチャンネル / 遺伝子操作 / 中間細胞 / 難聴 / 可逆性 / NKCC1 / 可逆性難聴 / 内耳 / 内リンパ / 聴覚

Outline of Final Research Achievements

The goal of the present study is to reveal mechanisms that cause variable inner ear dysfunction. We focused on the role of the stria vascularis of the cochlea, and we generated two animal models for which hearing can be repeatedly controlled at will to model variable hearing loss. One transgenic mouse line is the line that demonstrates inducible and reversible specific gene knockdown of an ion transporter by antibiotic administration and disadministration. Another transgenic mouse line is the line of which stria vascularis function is disturbed quickly using optogenetics. We demonstrated that these transgenic mice lines provided mouse models for the study of variable hearing loss and that the stria vascularis is involved in the mechanisms of variable hearing loss.

Research Products

• [Journal Article] Hearing Loss Controlled by Optogenetic Stimulation of Nonexcitable Nonglial Cells in the Cochlea of the Inner Ear (2017)
  • Author(s): Sato MP, et al.
  • Journal Title: Frontiers in Molecular Neuroscience Volume: 10 Pages: 300-300
  • DOI: 10.3389/fnmol.2017.00300
  • Peer Reviewed / Open Access
• [Journal Article] Time-controllable Nkcc1 knockdown replicates reversible hearing loss in postnatal mice. (2017)
  • Author(s): Watabe T, Xu M, Watanabe M, Nabekura J, Higuchi T, Hori K, Sato MP, Nin F, Hibino H, Ogawa K, Masuda M, Tanaka KF
  • Journal Title: Science Reports Volume: 7 Issue: 1 Pages: 13605-14007
  • DOI: 10.1038/s41598-017-13997-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Nkcc1の可逆的発現調節による蝸牛形態と機能変化の分析 (2017)
  • Author(s): 渡部 高久、増田 正次、小川 郁
  • Organizer: Nkcc1の可逆的発現調節による蝸牛形態と機能変化の分析
• [Presentation] Transient Induction of Deafness by Optogenesis Targeting the Endocochlear Potential in the Inner Ear (2016)
  • Author(s): Sato M; Nin F; Higuchi T; Uetsuka S; Yoshida T; Masuda M; Watabe T; Kanzaki S; Ogawa K; Doi K; Takebayashi H; Tanaka K; Hibino H
  • Organizer: THE 39TH ANNUAL MIDWINTER MEETING OF THE ARO
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2016-02-19
  • Int'l Joint Research
• [Remarks] 杏林大学 医学部 教員紹介 増田 正次
  • URL: http://www.kyorin-u.ac.jp/univ/faculty/medicine/education/staff/detail/?id=med33016