Relation of viral infection during pregnancy to hearing-impaired children
| Project/Area Number |
15K10771
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | National Center for Child Health and Development |
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Principal Investigator |
Morimoto Noriko 国立研究開発法人国立成育医療研究センター, 感覚器・形態外科部, 医長 (40286555)
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| Co-Investigator(Kenkyū-buntansha) |
宮入 烈 国立研究開発法人国立成育医療研究センター, 生体防御系内科部, 医長 (10296626)
齋藤 昭彦 新潟大学, 医歯学系, 教授 (30531389)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAMURA Hiroyuki 国立成育医療研究センター, 母児感染研究室長 (70256866)
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| Research Collaborator |
IZUMITA Ryouhei 新潟大学, 医学部小児科学, 大学院生
EDAYOSHI Mina 国立成育医療研究センター, 感染科, 研究助手
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 胎盤感染 / 遅発性難聴 / ウイルス感染 / 先天性風疹症候群 / RNAウイルス / 乾燥臍帯 / 臍帯 / 母胎胎内感染 / 先天性ウイルス感染 / 進行性難聴 / PCR / ウイルス胎盤感染 / 母胎感染 |
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| Outline of Final Research Achievements |
It is difficult to diagnose after long time passes whether it is infected during pregnancy.
Rubella virus is an RNA virus, so it is said to be more unstable and difficult to extract than DNA virus. We established a method to reliably identify rubella virus RNA from dry umbilical cord by RT-PCR method. As a result, it was possible to establish a procedure with sensitivity of 80% and specificity of 100%. The cause of false negatives is the possibility that the symptoms are light, the viral RNA content is low, and the possibility that it has deviated from the detection area due to RNA fragmentation. It will be necessary to raise invention and reproducibility to raise sensitivity more in future.
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Report
(4 results)
Research Products
(9 results)
