| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We have identified ZBTB10 as a candidate of novel deafness gene from a patient with cochlear nerve hypoplasia using whole exome analysis. ZBTB10 appeared to be expressed in the cochlear nerve. ZBTB10 expression was increased during nerve elongation of neuroblastoma NB1 cells. Zbtb10mut/+ mouse generated by CRISPR/Cas9 showed normal hearing. Low reproduction rate and anatomical observation suggests that the gene also affect proper function of ovary. All the Zbtb10mut/mut and half the number of Zbtb10mut/+ embryos seemed to be dead by the embryonic day 14.5 (E14.5). Zbtb10 appeared to be critical for early embryogenesis. We are now conducting histochemical study focusing on development of the brain, otocyst, and primordial germ line cells at E9.5. We will accumulate, analyze the data and report the results to the public soon.
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