basic research of SIRT1 activator for curative treatment of allergic rhinitis

Research Project

Project/Area Number	15K10775
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Otorhinolaryngology
Research Institution	Hokkaido University
Principal Investigator	Nakamaru Yuji 北海道大学, 医学研究院, 准教授 (20344509)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	アレルギー学 / ヒストンアセチル化酵素 / アレルギー性鼻炎 / TSLP
Outline of Final Research Achievements	To elucidate histone deacetylase for production of TSLP in patients with allergic rhinitis we used Beas-2B cells. After those cells were confluent, the inhibitor of histone deacetylase was added. Thirty minutes after the inhibitor was added, we stimulated the cells with ODN2006. Twenty four hours later, those cells were collected and the amout of TSLP mRNA was evaluated. We found that the inhibitor of histone deacetylase increased the amount of TSLP with dose-dependent manner.　On the other hand,the activator of histone deacetylase decleased the amount of TSLP with dose-dependent manner.

Report

(4 results)