The role of HRF in pollinosis

• Project/Area Number: 15K10794
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Hokkaido University (2016-2017); Institute of Physical and Chemical Research (2015)
• Principal Investigator: KASHIWAKURA Jun-ichi 北海道大学, 薬学研究院, 講師 (90373290)
• Research Collaborator: KAWAKAMI Toshiaki 理化学研究所, 統合生命医科学研究センター, チームリーダー (60143418) ; ANDO Tomoaki 順天堂大学, 医学研究科, 助教 (10724669)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ヒスタミン遊離因子（HRF) / マスト細胞 / 花粉症 / アレルギー性鼻炎 / HRF / 好塩基球 / IgE

Outline of Final Research Achievements

It is reported that Histamine-Releasing Factor (HRF) binds to a subset of IgE/IgG and is involved in mast cell-dependent allergic reactions. Howevere, it is still unknown whether HRF is involved in other allergic diseases such as pollinosis. The purpose of this study is to investigate the role of HRF in allergic rhinitis/pollinosis. It was shown that only HRF dimer has ability to stimulate HRF-reactive IgE-bound mast cells. Using ragweed pollen-induced pollinosis mouse model, it was observed that HRF treatment inhibited ragweed pollen-induced pollinosis, suggesting that HRF is involved in the allergic rhinitis/pollinosis.

Research Products

• [Journal Article] Crystal structures of murine and human Histamine-Releasing Factor (HRF/TCTP) and a model for HRF dimerisation in mast cell activation (2018)
  • Author(s): Dore Katy A.、Kashiwakura Jun-ichi、McDonnell James M.、Gould Hannah J.、Kawakami Toshiaki、Sutton Brian J.、Davies Anna M.
  • Journal Title: Molecular Immunology Volume: 93 Pages: 216-222
  • DOI: 10.1016/j.molimm.2017.11.022
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Role of Histamine-releasing Factor in Allergic Inflammatory Reactions (2017)
  • Author(s): 柏倉 淳一、安藤 智暁、川上 敏明
  • Journal Title: YAKUGAKU ZASSHI Volume: 137 Issue: 5 Pages: 517-521
  • DOI: 10.1248/yakushi.16-00239-3
  • NAID: 130005631392
  • ISSN: 0031-6903, 1347-5231
  • Year and Date: 2017-05-01
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Frontiers in Researches of Allergy (2017)
  • Author(s): Nishida K and Kashiwakura JI
  • Journal Title: YAKUGAKU ZASSHI Volume: 137 Issue: 5 Pages: 493-494
  • DOI: 10.1248/yakushi.16-00239-F
  • NAID: 130005631398
  • ISSN: 0031-6903, 1347-5231
  • Year and Date: 2017-05-01
  • Peer Reviewed
• [Journal Article] Histamine-releasing factor enhances food allergy (2017)
  • Author(s): Ando Tomoaki、Kashiwakura Jun-ichi、Itoh-Nagato Naoka、Yamashita Hirotaka、Baba Minato、Kawakami Yu、Tsai Shih Han、Inagaki Naoki、Takeda Kiyoshi、Iwata Tsutomu、Shimojo Naoki、Fujisawa Takao、Nagao Mizuho、Matsumoto Kenji、Kawakami Yuko、Kawakami Toshiaki
  • Journal Title: Journal of Clinical Investigation Volume: 127 Issue: 12 Pages: 4541-4553
  • DOI: 10.1172/jci96525
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Expression of TCTP-Related Genes (2017)
  • Author(s): Kashiwakura Jun-ichi、Kawkami Toshiaki
  • Journal Title: J. Genet. Disor. Genet. Rep Volume: 6 Pages: 2-2
  • Peer Reviewed / Int'l Joint Research
• [Presentation] アレルギー炎症反応におけるヒスタミン遊離因子の役割 (2016)
  • Author(s): 柏倉淳一
  • Organizer: 第136回日本薬学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-03-28
  • Invited