Mutational analysis on PI3K pathway in head and neck squamous cell carcinoma and its application to clinical sequence

• Project/Area Number: 15K10802
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: ASAKAGE Takahiro 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (50361481)
• Co-Investigator(Kenkyū-buntansha): 安藤 瑞生 東京大学, 医学部附属病院, 助教 (60511467)
• Co-Investigator(Renkei-kenkyūsha): SAITO Yuki 東京大学, 耳鼻咽喉科, 助教 (40611009) ; OMURA Go 国立がん研究センター中央病院, 頭頸部外科, 医員 (00601139)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 頭頸部癌 / 扁平上皮癌 / 免疫染色 / 遺伝子解析 / p16 / 次世代シークエンサー / 遺伝子変異 / シークエンス / ゲノムマーカー

Outline of Final Research Achievements

Newly diagnosed 108 patients with head and neck squamous cell carcinoma were included Oral/oropharyngeal/hypopharyngeal/laryngeal/sinonasal cancer were included 31/22/33/7/15 patients respectively. These age ranges from 38 to 96 years old, 89 males and 19 females. Stage I / II / III / IV were 25 cases / 20 cases / 13 cases / 45 cases respectively. For oropharyngeal cancer, p16 immunostaining, which is a slow-gate marker for HPV infection, was performed. Of 22 patients with oropharyngeal cancer, p16 positive was 8 cases, p16 negative 5 cases, unknown was 9 cases. 100 DNA samples were extracted.The facility change of the research representative and the study abroad of the person in charge of practice overlapped, causing delay in the execution of the research. Currently, gene analysis is in progress.

Research Products

• [Journal Article] The prognostic value of TP53 mutations in hypopharyngeal squamous cell carcinoma. (2017)
  • Author(s): Omura G, Ando M, Ebihara Y, Saito Y, Kobayashi K, Fukuoka O, Akashi K, Yoshida M, Asakage T, Yamasoba T.
  • Journal Title: BMC Cancer. Volume: 17 Issue: 1 Pages: 898-898
  • DOI: 10.1186/s12885-017-3913-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prognostic value of lymphovascular invasion of the primary tumor in hypopharyngeal carcinoma after total laryngopharyngectomy (2017)
  • Author(s): Saito Yuki、Omura Go、Yasuhara Kazuo、Rikitake Ryoko、Akashi Ken、Fukuoka Osamu、Yoshida Masafumi、Ando Mizuo、Asakage Takahiro、Yamasoba Tatsuya
  • Journal Title: Head & Neck Volume: 39 Issue: 8 Pages: 1535-1543
  • DOI: 10.1002/hed.24705
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Association of the upregulated expression of focal adhesion kinase with poor prognosis and tumor dissemination in hypopharyngeal cancer. (2016)
  • Author(s): Omura G, Ando M, Saito Y, Kobayashi K, Yoshida M, Ebihara Y, Kanaya K, Fujimoto C, Sakamoto T, Kondo K, Asakage T, Yamasoba T
  • Journal Title: Head Neck. Volume: Published on line Issue: 8 Pages: 1164-1169
  • DOI: 10.1002/hed.24176
  • Peer Reviewed
• [Journal Article] Mutational Landscape and Antiproliferative Functions of ELF Transcription Factors in Human Cancer. (2016)
  • Author(s): Ando M, Kawazu M, Ueno T, Koinuma D, Ando K, Koya J, Kataoka K, Yasuda T, Yamaguchi H, Fukumura K, Yamato A, Soda M, Sai E, Yamashita Y, Asakage T, Miyazaki Y, Kurokawa M, Miyazono K, Nimer SD, Yamasoba T, Mano H.
  • Journal Title: Cancer Research Volume: 未定
  • Peer Reviewed