| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The purpose of this study was to regenerate tracheal cartilage with a function as frame using chondrocytes and perichondrial cells. Rabbits were used as experimental animals. Under general anesthesia, outer portion of costal cartilage was resected, and inner portion of the cartilage was left to avoid pneumothorax. Chondrocytes and perichondrial cells were collected by treating minced cartilage and perichondrium with an enzyme. The chondrocytes and the perichondrial cells were suspended in solution containing type 1 collagen. The suspension with the chondrocytes was injected into collagenous sponge of artificial trachea that had been used in clinical, and the suspension with the perichondrial cells was applied to the surface of the artificial trachea. The suspensions were gelated in an incubator. The bioengineered trachea with the cells was autografted to the defect of the trachea.
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