| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2018: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2017: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2016: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
|
|---|
| Outline of Final Research Achievements |
Canine Adipose Derived Regenerative Cells (ADRCs) combined with the autologous fat was injected into the canine vocal folds to prevent the post-operative fat absorption. At the side of ADRCs injection, larger fat graft area and more vascular endothelial cells were observed compared to the control side. ADRCs produced more hepatocyte growth factor (HGF) than normal fat tissue. HGF-producing ability of ADRCs was able to confirm by qPCR, but the multi-differentiation ability was not able to confirm by the induced differentiation. Good vocal fold vibration had seen with ADRCs injection group according to the blowing examination.
The pathological reproduction effect of the epithelium and muscular layer was not clear. However, hyaluronic acid production could confirm in lamina propria. The regenerate lamina propria thickened and detected that the sparse collagen fiber and hyperplasia of elastic fiber.
|
