| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Outline of Final Research Achievements |
The stem cell system in the maculae flavae of the human vocal fold mucosa was investigated.
Cells in the human maculae flavae expressed the major makers for bone marrow derived circulating fibrocytes. The cultured cells in the human maculae flavae were undifferentiated and express proteins of all three germ layers. Using mesenchymal stem cell growth medium, the subcultured cells formed a colony-forming unit and cell division was an asymmetric self-renewal, indicating these cells are mesenchymal stem cells or stromal stem cells in the bone marrow. A proper microenvironment in the maculae flavae of the human vocal fold mucosa is necessary to be effective as a stem cell niche maintaining the stemness of the contained putative stem cells. The results of this study are consistent with the hypothesis that the cells in the human maculae flavae are putative stem cells of the human vocal fold mucosa.
|
