| Project/Area Number |
15K10857
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中林 征吾 旭川医科大学, 大学病院, 診療助教 (00451469)
坪井 尚子 旭川医科大学, 大学病院, 医員 (40548848)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 緑内障 / 濾過手術 / 創傷治癒 / 緑内障手術 / 眼圧 / MCP-1 / マクロファージ / 線維柱帯細胞 / 細胞外マトリクス / 結膜線維芽細胞 |
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| Outline of Final Research Achievements |
Previous reports have revealed that high aqueous MCP-1 level is related to poor surgical outcome of glaucoma filtration surgery. Therefore, we focused on the effect of MCP-1 on wound healing processes in glaucoma filtration bleb (bleb). Firstly, we applied MCP-1 on the bleb for a long period and investigated its effects on scar formation using rabbit models. As a result, the accumulation of inflammatory cells were observed. Second, we conducted in vitro experiments in that human conjunctival fibroblasts (HConF) and macrophages were co-cultured, and found that significant upregulation of α-SMA in HConF. Taken together, it was indicated that aqueous MCP-1 escape into the bleb after surgery for a long period and causes monocyte-macrophage recruitment, resulting in promoting wound healing reaction in the bleb. The results of current study were one of the important results in elucidating the mechanism of aqueous MCP - 1 on wound healing of the bleb after glaucoma filtration surgery.
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