| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Our group had established a stable culture method of polarized RPE by using the human commercial available RPE cell lines on Transwell. We had investigated drug permeability of anti-VEGF antibody which were currently used clinically with our polarized RPE, and found that the permeability ratio differs depending on the drug. We also analyzed the difference in response by TNF-α stimulation with or without polarity and found the ability of VEGF secretion and the interaction of intracellular signal were different depending on the polarity.
In the image analysis, there are structural changes of the choroid in eyes with CSC, the larger hyperreflective area change in the inner choroid and the larger hyporeflective area change in the outer choroid by using binarization methods. Most importantly, the quantification of the structural components of the choroid in the OCT B-scan images has extracted new information which should contribute to the determination of the mechanism of CSC.
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