Observation of corneal and scleral fibroblast network by using FIB/SEM
| Project/Area Number |
15K10886
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kurume University |
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Principal Investigator |
Hirata Akira 久留米大学, 医学部, 客員准教授 (60295144)
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| Co-Investigator(Kenkyū-buntansha) |
太田 啓介 久留米大学, 医学部, 准教授 (00258401)
中村 桂一郎 久留米大学, 医学部, 教授 (20172398)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 強膜 / 角膜 / 線維芽細胞 / 内境界膜 / FIB/SEM / 黄斑円孔 / 網膜前膜 |
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| Outline of Final Research Achievements |
To observe the three-dimensional structure of the mouse scleral fibroblasts by focused ion beam/scanning electron microscopy (FIB/SEM). Four eyes from C57BL/6J mice were fixed using a mixture of glutaraldehyde and formaldehyde. The sclera was cut out at the equatorial portion and at the posterior pole, and postfixed with potassium ferrocyanide, osmium, thiocarbohydrazide, uranyl acetate and lead aspartate. Specimens were then dehydrated and embedded in an epoxy resin. Serial block face images were obtained using FIB/SEM. Three-dimensional image reconstruction and segmentation of the image stack were created.
Scleral fibroblasts were arranged in collagenous layers. The cells frequently showed a cellular connection with the neighboring cells and formed cellular networks. Compared with equatorial fibroblasts, there was a more complicated cellular arrangement of the posterior scleral fibroblasts. Scleral fibroblasts connect with neighboring cells to form three-dimensional cellular networks.
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Report
(4 results)
Research Products
(4 results)
