| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
To investigate the physiological function of mitochondrial DNA in tear and serum from the patient of ocular diseases with various etiologies. The tears and serum were collected. The relationship between serum mitochondrial DNA (mtDNA) concentration and those pathophysiology were investigated. MtDNA was isolated and mtDNA level of tear and serum were measured real time-PCR. To investigate the immunological functions, human primary CD14-positive monocyte was used to measure the concentration of inflammatory mediators by using enzyme-linked immunosorbent assay after co-incubation with serum of patients in culture media. Results: The elevation of mtDNA was observed in tears of patients with cornea diseases such as dry eye, keratoconus, and limbal stem cell deficiency. However, the concentration of mtDNA from tear did not show a significant increase of in all ocular surface diseases. Cytokine analysis showed that tear of keratoconus patient had the immunological functions.
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