Roles of mitochondrila expression in ocular inflammation
| Project/Area Number |
15K10891
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Ophthalmology
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
Noriyasu Hashida 大阪大学, 医学系研究科, 特任講師(常勤) (30456959)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
川崎 諭 大阪大学, 医学系研究科, 特任准教授(常勤) (60347458)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | バイオマーカー / 慢性炎症 / ミトコンドリアDNA / ミトコンドリア |
|---|
| Outline of Final Research Achievements |
To investigate the physiological function of mitochondrial DNA in tear and serum from the patient of ocular diseases with various etiologies. The tears and serum were collected. The relationship between serum mitochondrial DNA (mtDNA) concentration and those pathophysiology were investigated. MtDNA was isolated and mtDNA level of tear and serum were measured real time-PCR. To investigate the immunological functions, human primary CD14-positive monocyte was used to measure the concentration of inflammatory mediators by using enzyme-linked immunosorbent assay after co-incubation with serum of patients in culture media. Results: The elevation of mtDNA was observed in tears of patients with cornea diseases such as dry eye, keratoconus, and limbal stem cell deficiency. However, the concentration of mtDNA from tear did not show a significant increase of in all ocular surface diseases. Cytokine analysis showed that tear of keratoconus patient had the immunological functions.
|
Report
(4 results)
Research Products
(13 results)
-
[Journal Article] Association of ABCG1 With Neovascular Age-Related Macular Degeneration and Polypoidal Choroidal Vasculopathy in Chinese and Japanese.2017
Author(s)
Ma L, Liu K, Tsujikawa M, Chen H, Brelen ME, Chan VC, Lai TY, Sayanagi K, Hara C, Hashida N, Tam PO, Young AL, Chen W, Nishida K, Pang CP, Chen LJ.
-
Journal Title
Invest Ophthalmol Vis Sci.
Volume: 57
Issue: 13
Pages: 5758-5763
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
[Journal Article] Ethnic specific association of the CAV1/CAV2 locus with primary open-angle glaucoma.2016
Author(s)
Rong SS, Chen LJ, Leung CK, Matsushita K, Jia L, Miki A, Chiang SW, Tam PO, Hashida N, Young AL, Tsujikawa M, Zhang M, Wang N, Nishida K, Pang CP.
-
Journal Title
Sci Rep.
Volume: 6
Issue: 1
Pages: 27837-27837
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
