Development and clinical application of rapid measurement of aqueous humor vascular endothelial growth factor

• Project/Area Number: 15K10899
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Jichi Medical University
• Principal Investigator: Takahashi Hidenori 自治医科大学, 医学部, 准教授 (10361479)
• Co-Investigator(Kenkyū-buntansha): 吉田 淳 自治医科大学, 医学部, 講師 (10420944) ; 井上 裕治 自治医科大学, 医学部, 講師 (10436488) ; 川島 秀俊 自治医科大学, 医学部, 教授 (30169718) ; 富永 聡子 自治医科大学, 医学部, 助教 (50748093) ; 池袋 一典 東京農工大学, 工学部, 教授 (70251494)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 超高感度測定 / 眼内炎症性サイトカイン / 微量濃度迅速測定 / 血管内皮増殖因子 / 眼医工学 / 検査・診断システム

Outline of Final Research Achievements

We developed the ultra high sensitive measurement of vascular endothelial growth factor only 10 pg/mL. This measurement is a novel high sensitive measurement and has widely general purpose, such as other cytokines, tumor markers, bacteria, tumor cells, and environmental hormone. The measurement enables rapid measurement of disease markers and bacteria, grasps patients' condition rapidly and easily, and enable early diagnosis and appropriate treatment to the patients. Other than medicine, this study results are useful to wide scientific field which needs high sensitive measurements, such as environmental hormone and general chemistry. In the ophthalmic field, we measured aqueous inflammatory cytokine concentrations and reported the pathogenesis of age-related macular degeneration and retinal vein occlusion.

Research Products

• [Int'l Joint Research] Singapore National Eye Centre/Singapore Eye Research Institute/Duke NUS Medical School(Singapore)
• [Journal Article] Changes in Multiple Cytokine Concentrations in the Aqueous Humor of Neovascular Age-related Macular Degeneration after Two Months of Ranibizumab Therapy (2018)
  • Author(s): Sakamoto S, Takahashi H, Tan X, Inoue Y, Nomura Y, Arai Y, Fujino Y, Kawashima H, Yanagi Y
  • Journal Title: Br J Ophthalmol Volume: 102 Issue: 4 Pages: 448-454
  • DOI: 10.1136/bjophthalmol-2017-310284
  • Peer Reviewed
• [Journal Article] Visual acuity loss associated with excessive “dry macula” in exudative age-related macular degeneration (2018)
  • Author(s): Takahashi H, Inoue Y, Tan X, Inoda S, Sakamoto S, Arai Y, Yanagi Y, Fujino Y, Kawashima H
  • Journal Title: Clin Ophthalmol Volume: 12 Pages: 369-375
  • DOI: 10.2147/opth.s151999
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A High-salt Diet Enhances Leukocyte Adhesion in Association with Kidney Injury in Young Dahl Salt-sensitive Rats. (2017)
  • Author(s): Takahashi H, Nakagawa S, Wu Y, Kawabata Y, Numabe A, Yanagi Y, Tamaki Y, Uehara Y, Araie M
  • Journal Title: Hypertens Res Volume: 40 Issue: 11 Pages: 912-920
  • DOI: 10.1038/hr.2017.31
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Applying artificial intelligence to disease staging: Deep learning for improved staging of diabetic retinopathy. (2017)
  • Author(s): Takahashi H, Tampo H, Arai Y, Inoue Y, Kawashima H
  • Journal Title: PLoS One Volume: 12 Issue: 6 Pages: e0179790-e0179790
  • DOI: 10.1371/journal.pone.0179790
  • Peer Reviewed / Open Access
• [Journal Article] Changes of outer retinal microstructures after photodynamic therapy for chronic central serous chorioretinopathy (2017)
  • Author(s): Shimizu Kimiko、Hashimoto Yohei、Azuma Keiko、Nomura Yoko、Obata Ryo、Takahashi Hidenori、Yanagi Yasuo
  • Journal Title: Clinical Ophthalmology Volume: Volume 11 Pages: 1505-1512
  • DOI: 10.2147/opth.s139288
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural regulation by a G-quadruplex ligand increases binding abilities of G-quadruplex-forming aptamers (2016)
  • Author(s): Tsukakoshi K, Ikuta Y, Abe K, Yoshida W, Iida K, Ma Y, Nagasawa K, Sode K, Ikebukuro K.
  • Journal Title: Chem Commun (Camb) Volume: 52(85) Issue: 17 Pages: 12646-12649
  • DOI: 10.1039/c0cc05079b
  • Peer Reviewed
• [Journal Article] Vascular Endothelial Growth Factor (VEGF) Concentration Is Underestimated by Enzyme-Linked Immunosorbent Assay in the Presence of Anti-VEGF Drugs. (2016)
  • Author(s): Takahashi H, Nomura Y, Nishida J, Fujino Y, Yanagi Y, Kawashima H
  • Journal Title: Investigative Ophthalmology & Visual Science Volume: 57 Issue: 2 Pages: 462-466
  • DOI: 10.1167/iovs.15-18245
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Aqueous humor inflammatory cytokine levels in chronic retinal vein occlusion (2015)
  • Author(s): Inoda S, Takahashi H, Tan X, Kakinuma N, Nomura Y, Sakamoto S, Arai Y, Fujino Y, Kawashima H, Yanagi Y
  • Organizer: The Association for Research in Vision and Ophthalmology
  • Place of Presentation: Denver, Colorado
  • Year and Date: 2015-05-03
  • Int'l Joint Research
• [Presentation] 網膜静脈閉塞症慢性期における前房中炎症性サイトカイン濃度 (2015)
  • Author(s): 伊野田悟，高橋秀徳，譚雪，垣沼奈津子，野村陽子，坂本晋一，新井悠介，富永聡子，佐藤彩，藤野雄次郎，川島秀俊，柳靖雄
  • Organizer: 第119回日本眼科学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2015-04-16
• [Remarks] 高橋 秀徳 - 研究者 - researchmap
  • URL: https://researchmap.jp/hidenoritakahashi/