Anti-inflammatory effects of Novel NF-kB inhibitor, DHMEQ on in vitro and in vivo model of ocular inflammation
| Project/Area Number |
15K10901
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Nakayama Makiko 杏林大学, 医学部, 助教 (30736278)
Keino Hiroshi 杏林大学, 医学部, 准教授 (90328211)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 眼炎症 / ぶどう膜炎 |
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| Outline of Final Research Achievements |
Dehydroxymethylepoxyquinomicin (DHMEQ) is a novel nuclear factor (NF)-kB inhibitor. We investigated whether DHMEQ can modulate the proinflammatory cytokine induced expression of intracellular adhesion molecule (ICAM)-1 and chemokines in a human retinal pigment epithelial (RPE) cell line (ARPE-19). In addition, we investigated whether intravitreal injection of DHMEQ was effective for suppression of experimental autoimmune uveoretinitis (EAU). DHMEQ effectively down-regulated the expression of ICAM-1 in TNF-alpha stimulated ARPE-19 cells. Furthermore, DHMEQ suppressed the production of both IL-8 and MCP-1. However, there was no significant difference in EAU clinical score between control eyes and DHMEQ-treated eyes in EAU-induced rats. These results suggest that DHMEQ may play a role in reducing ICAM-1 and chemokine mediated inflammatory disorders in the eye, however further study is warranted for clinical application of DHMEQ for autoimmune uveitis.
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] Interobserver Agreement Among Uveitis Experts on Uveitic Diagnoses: The Standardization of Uveitis Nomenclature Experience.2018
Author(s)
Jabs DA, Dick A, Doucette JT, Gupta A, Lightman S, McCluskey P, Okada AA, Palestine AG, Rosenbaum JT, Saleem SM, Thorne J, Trusko B.
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Journal Title
Am J Ophthalmol.
Volume: 186
Pages: 19-24
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Behcet Uveitis.2017
Author(s)
Cunningham ET Jr, Tugal-Tutkun I, Khairallah M, Okada AA, Bodaghi B, Zierhut M.
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Journal Title
Ocul Immunol Inflamm.
Volume: 25
Pages: 2-6
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Behcet Uveitis2017
Author(s)
Cunningham ET Jr, Tugal-Tutkun I, Khairallah M, Okada AA, Bodaghi B, Zierhut M
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Journal Title
Ocul Immunol Inflamm.
Volume: 25
Issue: 1
Pages: 2-6
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Investigation of the association between IL10 gene polymorphisms and Vogt-Koyanagi-Harada disease in a Japanese population.2016
Author(s)
Kaori Higashia, Akira Meguroa, Masaki Takeuchiab, Takahiro Yamanea, Nobuyoshi Kitaichic, Yukihiro Horiec, Kenichi Nambad, Shigeaki Ohnod, Kumiko Nakaoe, Taiji Sakamotoe, at el.
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Journal Title
Ophthalmic Genetics
Volume: -
Issue: 2
Pages: 187-189
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Dehydroxymethylepoxyquinomicin, a novel nuclear factor-κB inhibitor, reduces chemokines and adhesion molecule expression induced by IL-1β in human corneal fibroblasts.2015
Author(s)
Inokawa S, Watanabe T, Keino H, Sato Y, Hirakata A, Okada AA, Fukuda K, Fukushima A, Umezawa K.
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Journal Title
Graefes Arch Clin Exp Ophthalmol.
Volume: 253
Issue: 4
Pages: 557-563
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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