Immunological properties of corneal endothelial cells derived from human induced pluripotent stem cells
| Project/Area Number |
15K10902
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Keio University |
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Principal Investigator |
Shin Hatou 慶應義塾大学, 医学部(信濃町), 特任講師 (70327542)
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| Research Collaborator |
Shimmura Shigeto 慶應義塾大学, 医学部, 准教授
Fujii Shota 慶應義塾大学, 医学部
Yoshida Satoru 医薬基盤健康栄養研究所, 難治性疾患研究開発支援センター, プロジェクトマネージャー
Sugita Sunao 理化学研究所, 多細胞システム形成研究センター, 副プロジェクトリーダー
Takahashi Masayo 理化学研究所, 多細胞システム形成研究センター, プロジェクトリーダー
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | iPS細胞 / 神経堤細胞 / 角膜内皮細胞 / 免疫寛容 / T細胞 / TGF-β / 制御性T細胞 |
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| Outline of Final Research Achievements |
Corneal endothelial cells (CECs) are developed from neural crest cells (NCCs) and may play a role in anterior chamber associated immune deviation, but exact mechanism is yet to be known. In this study, we focused on immunological properties of iPS-cell-derived-NCCs (iPSC-NCCs), because of its high efficiency of induction. iPSC-NCCs were hypoimmunogenic and had immunosuppressive properties in vitro. iPSC-NCCs greatly inhibited T-cell activation (cell proliferation, production of inflammatory cytokines). iPSC-NCCs constitutively expressed TGF-β, and TGF-β produced by iPSC-NCCs played a critical role in T cell suppression. Hypoimmunogenic and immunosuppressive properties of NCCs may contribute to the realization of using stem cell-derived NCCs in cell-based medicine.
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Report
(4 results)
Research Products
(3 results)
