Spheroidal cultivation of the human limbal epithelial niche from small limbal tissue.
| Project/Area Number |
15K10907
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Ophthalmology
|
|---|
| Research Institution | Tokyo Dental College |
|---|
Principal Investigator |
HIGA KAZUNARI 東京歯科大学, 歯学部, 助教 (60398782)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | corneal limbal niche / 角膜上皮幹細胞 / ニッチ / スフェロイド / 幹細胞ニッシェ / 角膜輪部上皮 |
|---|
| Outline of Final Research Achievements |
To improve the cultivation method, we assumed a limited autologous tissue and performed spheroidal cultivation from human small limbal tissue. Uniformly small and round spheroids were formed even from small limbal tissue. Efficiency of spheroidal formation was differed in the derived site of the original limbus. Spheroids derived from the high spheroidal formation site showed the large colony forming efficiency and higher ratio of slow cycling cells. In addition, N-cadherin was infrequently-expressed in epithelial spheroid. Spheroids were maintained post-operative adaptation and epithelial proliferation in a limbal-deficient rabbit transplantation model.
|
Report
(4 results)
Research Products
(5 results)
