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Elucidation of favorable neuroblastoma factor and its application for the pathological control

Research Project

Project/Area Number 15K10921
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pediatric surgery
Research InstitutionHiroshima University

Principal Investigator

YAMAOKA EMI  広島大学, 自然科学研究支援開発センター, 研究員 (20403503)

Co-Investigator(Kenkyū-buntansha) 栗原 將  広島大学, 医歯薬保健学研究科(医), 助教 (40724894)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords小児がん / 神経芽腫 / 予後因子 / Neuroblasoma
Outline of Final Research Achievements

We performed functional analysis of DHRS3, CYP26A1 and NR0B1, highly expressed in favorable neuroblastoma transfected into neuroblastoma cell lines such as SKNSH, NH12, TGW, GOTO and NH6 with an overexpression and silensing system. DHRS3 and NR0B1 induced cell proliferation, cell cycle suppression, decreased tumorigenic ability.
In exhaustive transcriptome analysis using RNAseq, expression of genes related to cell cycle and cell proliferation was decreased. We found the group of genes involved in the suppression of TGF-βsignaling involved in the retinoid X receptor activation pathway, JNKsignaling, tumor suppression and promotion of tumor growth. Therefore, DHRS3 and NR0B1 may be associated with spontaneous degeneration of neuroblastoma.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2017 2016 2015

All Presentation (6 results)

  • [Presentation] DHRS3とNR0B1は神経芽腫細胞株の分化と増殖抑制を誘導する2017

    • Author(s)
      山岡絵美、久保陽子、檜山英三
    • Organizer
      日本癌学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 予後良好な神経芽細胞腫で高発現していたDHRS3, NR0B1, CYP26A1の機能解析2017

    • Author(s)
      山岡 絵美、金輪 真佐美、田川 浩美、福場 郁子、久保-林 陽子、古屋敷 なぎさ、阿部 裕子、平野 尚子、檜山 英三
    • Organizer
      日本分子生物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 小児肝がん細胞において高発現する膜蛋白ADAM32の意義2017

    • Author(s)
      深澤 賢宏、谷本 圭司、山岡 絵美、金輪 真佐美、廣橋 伸之、檜山 英三
    • Organizer
      日本分子生物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 予後良好な神経芽細胞腫で発現していた3遺伝子の機能解析2016

    • Author(s)
      山岡絵美
    • Organizer
      第75回日本癌学会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2016 Research-status Report
  • [Presentation] Analysis of differentiation inducible factor candidates in human neuroblastoma cell lines.2016

    • Author(s)
      山岡絵美
    • Organizer
      第39回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2016 Research-status Report
  • [Presentation] Functional analysis of three genes expressed in favorable neuroblastoma.2015

    • Author(s)
      Emi Yamaoka
    • Organizer
      第38回 日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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