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Development of new therapy using stem cells from human exfoliated deciduous teeth for liver failure

Research Project

Project/Area Number 15K10923
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pediatric surgery
Research InstitutionKyushu University

Principal Investigator

Hayashida Makoto  九州大学, 医学研究院, 共同研究員 (70452761)

Co-Investigator(Kenkyū-buntansha) 田口 智章  九州大学, 医学研究院, 教授 (20197247)
松浦 俊治  九州大学, 医学研究院, 講師 (10532856)
柳 佑典  九州大学, 大学病院, 助教 (30596664)
吉丸 耕一朗  九州大学, 大学病院, 助教 (60711190)
山座 孝義  九州大学, 歯学研究院, 准教授 (80304814)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords小児外科 / 幹細胞 / 肝不全 / 肝再生 / 幹細胞移植 / 再生医療 / 乳歯幹細胞 / 肝細胞移植
Outline of Final Research Achievements

We transplanted 1.0x10^6 SHEDs into the CCl4 induced liver failure-model mouse via spleen. Histlogical analysis showed that the fibrotic lesion was decreased and the necrotic lesion of hepatocyte was improved. We investigated the mechansims of these effects. It showed that the amelioration of activation of stellate cells lead to decrease the fibrotic lesion, and that the inhibition of the proliferation of immune cells such as macrophages and T lymphocytes resulted in the anti-inflammatory effect. Moreover, we succesfully established the culture methods to defferentiate SHED into hepatocyte-like cell in vitro. The SHED-derived hepatocyte also showed the anti-fibrotic and anti-inflammatory effect in the liver failure-model mouse. We next established the culture methods of SHEDs with Xeno-free culture for the usage in clinical stage.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (4 results)

All 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (3 results)

  • [Journal Article] 乳歯幹細胞を用いた肝再生研究2017

    • Author(s)
      栁 佑典
    • Journal Title

      小児外科

      Volume: 49 Pages: 567-571

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] 乳歯幹細胞を用いた肝再生研究の現状と展望2016

    • Author(s)
      栁 佑典
    • Organizer
      第52回日本周産期・新生児医学会学術集会
    • Place of Presentation
      富山
    • Year and Date
      2016-07-16
    • Related Report
      2016 Research-status Report
  • [Presentation] 乳歯肝細胞を用いた肝再生医療研究2016

    • Author(s)
      栁 佑典
    • Organizer
      第53回日本小児外科学会
    • Place of Presentation
      福岡
    • Year and Date
      2016-05-24
    • Related Report
      2016 Research-status Report
  • [Presentation] 肝移植に代わる治療手段としての乳歯歯髄幹細胞移植2016

    • Author(s)
      田口 智章
    • Organizer
      第15回日本再生医療学会
    • Place of Presentation
      大阪
    • Year and Date
      2016-03-17
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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