Searching for the novel biomarker of Hirschsprung disease: focusing on the mechanism of extrinsic nerve fiber development
| Project/Area Number |
15K10929
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Juntendo University |
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Principal Investigator |
MIYAHARA Katsumi 順天堂大学, 医学(系)研究科(研究院), 研究技師 (00420844)
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| Co-Investigator(Kenkyū-buntansha) |
山高 篤行 順天堂大学, 医学部, 教授 (40200703)
田中 奈々 順天堂大学, 医学部, 准教授 (50530656)
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| Research Collaborator |
TAKAHASHI Mirei
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ヒルシュスプルング病 / 腸管神経系 / アセチル化チューブリン / エンドセリンレセプターB / 腸管神経 / 感覚神経 / サブスタンス P / CGRP / 神経堤細胞 / 軸索ガイダンス分子 |
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| Outline of Final Research Achievements |
Tubulin acetylation is involved in the regulation and stability of microtubule structure and plays an important role in various neuronal functions. In this study, the relationship between the expression of acetylated α-tubulin (Ac-Tub) in enteric neurons of the endothelin receptor B-knockout (EDNRB KO) mouse, which is a mouse model of Hirschsprung disease (HD), and developmental abnormalities of the enteric nervous system (ENS) were examined.
Fluorescent immunohistochemical analysis with Ac-Tub antibody was performed to assess the expression pattern of Ac-Tub in neuronal cells, and the expression in the EDNRB KO embryos was found to be increased compared to that in the normal embryos. The results indicated that the change in Ac-Tub expression in the developing enteric neurons may be associated with developmental abnormalities related to the innervation of the ENS in HD.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Rectal mucosal dissection commencing directly on the anorectal line versus commencing above the dentate line in laparoscopy-assisted transanal pull-through for Hirschsprung's disease: Prospective medium-term follow-up2015
Author(s)
Miyano G, Koga H, Okawada M, Doi T, Sueyoshi R, Nakamura H, Seo S, Ochi T, Yamada S, Imaizumi T, Lane GJ, Okazaki T, Urao M, Yamataka A
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Journal Title
J Pediatr Surg
Volume: 50
Issue: 12
Pages: 2041-2043
DOI
Related Report
Peer Reviewed / Open Access
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