| Project/Area Number |
15K10940
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
UENO Masaki 香川大学, 医学部炎症病理, 教授 (30322267)
TAMAI Motogi 香川大学, 医学部形成外科, 助教 (90617276)
KOGURE Tetsukuni 香川大学, 医学部形成外科, 助教 (40437695)
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| Research Collaborator |
TANAKA Yoshio 香川大学, 医学部形成外科, 研究協力員 (50171806)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 再生医療 / 脂肪組織 / PRP / bFGF / chamber / 線維芽細胞様細胞 / 再生脂肪組織 / adipose flap / 血管茎 / fibrocyte / 脂肪細胞 / circulating fibricyte / 血管付加脂肪組織 / in vivo chamber / ダイレクト・リプログラミング / チャンバー / 多血小板血漿 / コラーゲンスポンジ / 徐放性 |
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| Outline of Final Research Achievements |
The present study clarified 1) the reproducibility of creating an adipose flap, 2) the time-course of adipogenesis, 3) the long-term stability of a generated adipose flap, and 4) the mechanism of adipogenesis in an in vivo chamber. The chamber was used as a protected space for tissue engineering, the vascular pedicle as a vascular source, the collagen sponge as a scaffold, and PRP and c-bFGF as growth factors.Results)The ratios of the vascular pedicle and adipose tissue in the generated tissue were 14.8%, 47% and 80% at 4, 8, and 12 weeks, respectively. The adipose flap in Group 12w maintained its volume and shape for five months following pedicle transfer. Conclusions:Adipose flaps were spontaneously generated at 12 weeks with reproducibility, and showed long-term stability. Fibroblast-like cells recruited from the vascular pedicle was thought to contribute to the adipogenesis in the tissue-engineering chamber.
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