Development of new therapy of protracted refractory skin ulcer by using micro biome

• Project/Area Number: 15K10941
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Ehime University
• Principal Investigator: Hideki Mori 愛媛大学, 医学系研究科, 助教 (60325389)
• Co-Investigator(Kenkyū-buntansha): 中岡 啓喜 愛媛大学, 医学部附属病院, 准教授 (30172266)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: マイクロバイオーム / HMGB1 / 創傷治癒 / 角化細胞 / 単層培養 / 創傷治癒モデル / 三次元培養表皮（LSE)

Outline of Final Research Achievements

S. epidermidis inhibited production of inflammatory cytokines induced by s. aureus in keratinocyte. High Mobility Group Box 1(HMGB1), which is one of nuclear protein, was strongly expressed in abscess of mouse ear induced by s. aureus, but not by s. epidermidis. Poly(I:C), which is synthesized dsRNA, induced inflammatory cytokines in keratinocyte. We found that reduced-HMGB1 suppressed poly(I:C) induced inflammation in keratinocyte.

Research Products

• [Journal Article] Reduced-HMGB1 suppresses poly(I:C)-induced inflammation in keratinocytes (2018)
  • Author(s): Hideki Mori, Masamoto Murakami, Teruko Tsuda, Kenji Kameda, Ryo Utsunomiya, Kana Masuda, Ken Shiraishi, Xiuju Dai, Mikiko Tohyama, Hiroki Nakaoka, Koji Sayama
  • Journal Title: Journal of DermatologicalScience Volume: 90 Issue: 2 Pages: 154-165
  • DOI: 10.1016/j.jdermsci.2018.01.007
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Newly discovered function of reduced-HMGB1 as an inflammatory suppressor in keratinocyte (2017)
  • Author(s): Mori H, Murakami M, Utsunomiya R, Masuda K, Shiraishi K, Dai X, Tohyama M, Sayama K.
  • Organizer: 47th Annual ESDR Meeting
  • Int'l Joint Research
• [Presentation] Another role of exogenous HMGB1 on poly(I:C)-induced inflammation in keratinocyte (2017)
  • Author(s): Mori H, Murakami M, Utsunomiya R, et al.
  • Organizer: 42nd annual meeting of the Japanese Society for investigative Dermatology
  • Int'l Joint Research
• [Presentation] Suppressive effect of HMGB1 on poly(I:C)-induced inflammation. (2016)
  • Author(s): 森 秀樹
  • Organizer: 欧州研究皮膚科学会
  • Place of Presentation: ドイツ・ミュンヘン
  • Year and Date: 2016-09-07
  • Int'l Joint Research
• [Presentation] 頭蓋骨再建に利用した人工材料の検討 (2015)
  • Author(s): 森 秀樹
  • Organizer: 日本形成外科学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2015-04-08