Practicability of end-to-side neurorrhaphy as Schwann cell provider to acellular nerve graft

• Project/Area Number: 15K10949
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Juntendo University
• Principal Investigator: HAYASHI Ayato 順天堂大学, 医学部, 教授 (10365645)
• Co-Investigator(Kenkyū-buntansha): 水野 博司 順天堂大学, 医学部, 教授 (80343606)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 神経再生 / 端側神経縫合 / 人工神経 / 無細胞化神経 / 神経移植 / シュワン細胞 / 脂肪組織由来幹細胞

Outline of Final Research Achievements

To improve usefulness of artificial nerve graft, we have proceeded the research to use end-to-side neurorrhaphy as Schwann cell provider to acellular nerve graft.
In this research, we compared the effect of end-to-side neurorrhaphy to the acellular nerve graft to injecting Adipose-derived stem cells(ASCs). Both procedures improved functional recovery after the graft. However, the difference between two procedures were not obvious and there was no synergic effect even combining them simultaneously. We try to conduct further research to find even more effective use of end-to-side neurorrhaphy as Schwann cell provider to acellular nerve graft in the future.

Research Products

• [Journal Article] Application of Kuhnt?Szymanowski Procedure to Lower Eyelid Margin Defect after Tumor Resection (2017)
  • Author(s): Hayashi Ayato、Mochizuki Mariko、Kamimori Tomoki、Horiguchi Masatoshi、Tanaka Rica、Mizuno Hiroshi
  • Journal Title: Plastic and Reconstructive Surgery - Global Open Volume: 5 Issue: 2 Pages: e1230-e1230
  • DOI: 10.1097/gox.0000000000001230
• [Journal Article] A synthetic leukotriene B4 receptor type 2 agonist accelerates the cutaneous wound healing process in diabetic rats by indirect stimulation of fibroblasts and direct stimulation of keratinocytes (2017)
  • Author(s): Luo, L., Tanaka, R., Kanazawa, S., Lu, F., Hayashi, A., Yokomizo, T.,Mizuno, H.
  • Journal Title: J Diabetes Complications Volume: 31 Issue: 1 Pages: 13-20
  • DOI: 10.1016/j.jdiacomp.2016.09.002
  • Peer Reviewed / Open Access
• [Journal Article] Successful Treatment of Infantile Hemangiomas With Propranolol in Low-Birth-Weight Infants (2017)
  • Author(s): Kado Makiko、Shimizu Azusa、Matsumura Takashi、Mochizuki Mariko、Mizuno Hiroshi、Hayashi Ayato
  • Journal Title: Journal of Craniofacial Surgery Volume: 28 Issue: 3 Pages: 789-793
  • DOI: 10.1097/scs.0000000000003542
• [Journal Article] Interleukin-6 stimulates Akt and p38 MAPK phosphorylation and fibroblast migration in non-diabetic but not diabetic mice (2017)
  • Author(s): Nishikai-Yan Shen Tsubame、Kanazawa Shigeyuki、Kado Makiko、Okada Kayoko、Luo Lin、Hayashi Ayato、Mizuno Hiroshi、Tanaka Rica
  • Journal Title: PLOS ONE Volume: 12 Issue: 5 Pages: e0178232-e0178232
  • DOI: 10.1371/journal.pone.0178232
  • Peer Reviewed / Open Access
• [Journal Article] Medial Canthal Reconstruction With Malar Advancement Flap Combined With Cutaneous Island Flap Created Using “Conventionally Discarded” Tissue (2017)
  • Author(s): Orgun Doruk、Hayashi Ayato
  • Journal Title: Dermatologic Surgery Volume: 印刷中 Issue: 9 Pages: 1-1
  • DOI: 10.1097/dss.0000000000001037
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] End-to-Side Neurorrhaphy as Schwann Cells Provider to Acellular Nerve Allograft and Its Suitable Application. (2016)
  • Author(s): Yoshizawa H, Senda D, Natori Y, Tanaka R, Mizuno H, Hayashi A
  • Journal Title: PLoS One Volume: 11 Issue: 12 Pages: e0167507-e0167507
  • DOI: 10.1371/journal.pone.0167507
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Fluorescent Intensity from the Transgenic Thy1-Yellow Fluorescent Protein 16 Mouse Correlates with the Amount of Regenerated Axons. (2016)
  • Author(s): Natori Y, Yoshizawa H, Senda D, Mizuno H, Hayashi A
  • Journal Title: Plastic and Reconstructive Surgery Global Open Volume: 4 Issue: 6 Pages: e758-e758
  • DOI: 10.1097/gox.0000000000000792
  • Peer Reviewed / Open Access
• [Journal Article] Levator lengthening technique using cartilage or fascia graft for paralytic lagophthalmos in facial paralysis (2016)
  • Author(s): Hayashi A, Yoshizawa H, Natori Y, Senda D, Tanaka R, Mizuno H.
  • Journal Title: Journal of Plastic, Reconstructive & Aesthetic Surgery Volume: 69 Issue: 5 Pages: 679-686
  • DOI: 10.1016/j.bjps.2016.01.010
  • Peer Reviewed / Open Access
• [Journal Article] Effectiveness of platysma muscle flap in preventing Frey syndrome and depressive deformities after parotidectomy (2016)
  • Author(s): Hayashi A, Mochizuki M, Suda S, Natori Y, Ando E, Yoshizawa H, Senda D, Tanaka R, Mizuno H
  • Journal Title: Journal of Plastic, Reconstructive & Aesthetic Surgery Volume: 69 Issue: 5 Pages: 663-672
  • DOI: 10.1016/j.bjps.2015.12.021
  • Peer Reviewed / Open Access
• [Journal Article] Experience and anatomical study of modified lenghthening temporalis myoplasty for established facial paralysis (2015)
  • Author(s): Hayashi A, Labbé D, Natori Y, Yoshizawa H, Kudo H, Sakai T and Mizuno H
  • Journal Title: J Plast Reconstr Aesthet Surg Volume: 68 Issue: 1 Pages: 63-70
  • DOI: 10.1016/j.bjps.2014.09.037
  • Peer Reviewed
• [Journal Article] Intraoperative use of indocyanine green fluorescence angiography during distally-based radial artery perforator flap for squamous cell carcinoma of the thumb (2015)
  • Author(s): Hayashi A, Yokoyama J, Sato R, Yoshizawa H, Tanaka R, Natori Y, Ohba S, Fukumura Y, Arakawa A, Mizuno H
  • Journal Title: Plast Reconstr Surg Glob Open. Volume: 3 Issue: 2 Pages: e310-e310
  • DOI: 10.1097/gox.0000000000000281
  • Peer Reviewed / Open Access
• [Journal Article] Conjunctival squamous cell carcinoma due to long-term placement of ocular prosthesis (2015)
  • Author(s): Hayashi A, Komoto M, Matsumura T, Horiguchi M, Tanaka R, Arakawa A, Mizuno H
  • Journal Title: Plast Reconstr Surg Glob Open. Volume: 3 Issue: 3 Pages: e325-e325
  • DOI: 10.1097/gox.0000000000000299
  • Peer Reviewed / Open Access
• [Journal Article] Massive myoepithelial carcinoma originating from the submandibular gland that was successfully treated with surgical excision using a part of the lengthened skin as a local flap (2015)
  • Author(s): Hayashi A, Yokoyama J, Sato R, Yoshizawa H, Tanaka R, Natori Y, Ohba S, Fukumura Y, Arakawa A, Mizuno H
  • Journal Title: Plast Reconstr Surg Glob Open. Volume: 3 Issue: 3 Pages: e329-e329
  • DOI: 10.1097/gox.0000000000000296
  • Peer Reviewed / Open Access
• [Journal Article] The availability of perifascial areolar tissue graft for deep cutaneous ulcer coverage (2015)
  • Author(s): Hayashi A, Komoto M, Tanaka R, Natori Y, Matsumura T, Horiguchi M, Matsuda N, Yoshizawa H, Mizuno H
  • Journal Title: J Plast Reconstr Aesthet Surg Volume: 68 Issue: 12 Pages: 1743-1749
  • DOI: 10.1016/j.bjps.2015.08.008
  • Peer Reviewed
• [Presentation] Basic research and clinical application of peripheral nerve regeneration in plastic and reconstructive surgeryEnd to side neurorrhaphy (2017)
  • Author(s): Hayashi A
  • Organizer: 16th Mae Fah Luang International Research Conference in Anti-Aging and Regenerative Medicine
  • Int'l Joint Research / Invited
• [Presentation] Combination of hypoglossal nerve transfer using an interposition nerve graft with end-to-side neurorrhaphy and masseter nerve transfer for the acute to subacute facial reanimation (2017)
  • Author(s): Hayashi A, Yoshizawa H, Senda D, Orgun D and Mizuno H
  • Organizer: 9th Congress of World Society for Reconstructive Microsurgery
  • Int'l Joint Research
• [Presentation] Modified Lengthening Temporalis Myoplasty with Intraoral Approach (2017)
  • Author(s): Hayashi A, Yoshizawa H, Senda D, and Mizuno H
  • Organizer: 13th International Facial Nerve Symposium
  • Int'l Joint Research
• [Presentation] Combination of hypoglossal nerve transfer using an interposition nerve graft with end-to-side neurorrhaphy and masseter nerve transfer for the acute to subacute facial reanimation (2017)
  • Author(s): Hayashi A, Yoshizawa H, Senda D, Orgun D and Mizuno H
  • Organizer: 13th International Facial Nerve Symposium
  • Int'l Joint Research
• [Presentation] 無細胞化神経に対するシュワン細胞付加法としての端側神経縫合の可能性 (2017)
  • Author(s): 林 礼人、吉澤秀和、千田大貴
  • Organizer: 第28回日本末梢神経学会学術集会
• [Presentation] Modified Lengthening Temporalis Myoplasty through an Intraoral approach (2016)
  • Author(s): Hayashi A, Yoshizawa H, Senda D, Mizuno H
  • Organizer: The 11th Congress of the Asian Pacific Craniofacial Association
  • Place of Presentation: 大阪
  • Year and Date: 2016-12-01
  • Int'l Joint Research
• [Presentation] 顔面神経麻痺に対する神経移行術の適応とその手技について (2016)
  • Author(s): 林 礼人、吉澤秀和、千田大貴、水野博司
  • Organizer: 第43回日本マイクロサージャリ―学会学術集会
  • Place of Presentation: 広島
  • Year and Date: 2016-11-17
• [Presentation] 端側神経縫合を用いた無細胞化神経へのシュワン細胞付加による移植神 経の長さによる遊走能の変化について (2016)
  • Author(s): 吉澤秀和、林 礼人、千田大貴、水野博司
  • Organizer: 第25回日本形成外科学会基礎学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2016-09-15
• [Presentation] 運動・知覚神経再生における短期的電気刺激の有用性について (2016)
  • Author(s): 名取悠平、林 礼人、吉澤秀和、千田大貴、水野博司
  • Organizer: 第25回日本形成外科学会基礎学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2016-09-15
• [Presentation] 我々の行ってきた急性期顔面神経麻痺に対する舌下神経縦二分割移行に ついて (2016)
  • Author(s): 林 礼人、西田匡伸、瀬野久和、井上雅博、岩田浩嗣、白澤友裕、 新井 一、栢森良二、小室裕造、梁井 皎
  • Organizer: 第59回日本形成外科学会総会・学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2016-04-13
  • Invited
• [Presentation] End-to-Side Neurorrhaphy as Schwann Cells Provider To Acellular Nerve Graft and its Effect (2016)
  • Author(s): Ayato Hayashi, Hidekazu Yoshizawa, Daiki Senda, Hiroshi Mizuno
  • Organizer: AMERICAN SOCIETY FOR PERIPHERAL NERVE 2016 ANNUAL MEETING
  • Place of Presentation: Scottsdale, Arizona, USA
  • Year and Date: 2016-01-15
  • Int'l Joint Research
• [Presentation] Suitable Application of End-to-Side Neurorrhaphy as Schwann Cells Provider to Acellular Nerve Graft (2016)
  • Author(s): Hidekazu Yoshizawa, Ayato Hayashi, Daiki Senda, Hiroshi Mizuno
  • Organizer: AMERICAN SOCIETY FOR PERIPHERAL NERVE 2016 ANNUAL MEETING
  • Place of Presentation: Scottsdale, Arizona, USA
  • Year and Date: 2016-01-15
  • Int'l Joint Research
• [Presentation] 無細胞化神経へのシュワン細胞付加法としての端側神経縫合とその応用について（第3報） (2015)
  • Author(s): 吉澤秀和、林礼人、千田大貴、水野博司
  • Organizer: 第24回日本形成外科学会基礎学術集会
  • Place of Presentation: 岩手県民会館
  • Year and Date: 2015-10-08