Investigating the changes in anti-coagulation ability in vascular endothelial cells during acute phase traumatic coagulopathy

• Project/Area Number: 15K10964
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Hokkaido University
• Principal Investigator: Hayakawa Mineji 北海道大学, 医学研究院, 講師 (10374282)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 播種性血管内凝固 / 外傷 / 凝固障害 / 血管内皮細胞 / トロンボモジュリン / 線溶 / 凝固 / 播種性血管内凝固症候群 / 止血

Outline of Final Research Achievements

In the present study, we tried to histologically elucidate the changes in thrombomodulin and glycocalyx in vascular endothelial cells. However, we could not detect significant changes in thrombomodulin and glycocalyx in vascular endothelial cells in DRAM-shock rat models. In these rat models, we identified microparticles, which activate the coagulation cascade, in the systemic circulation. Furthermore, the amounts of these microparticles were correlated with the severity of trauma.
Based on these findings, I formulated manuscripts using clinical data. Furthermore, I am currently formulating a manuscript based on the present study.

Research Products

• [Journal Article] Hyperfibrinolysis in severe isolated traumatic brain injury may occur without tissue hypoperfusion: a retrospective observational multicentre study (2017)
  • Author(s): Hayakawa Mineji、Kushimoto Shigeki、Ogura Hiroshi、Matsuoka Tetsuya、Morimura Naoto、Ishikura Hiroyasu、Kaneko Naoyuki、Nakamura Yoshihiko、Shiraishi Atsushi、Murata Kiyoshi
  • Journal Title: Critical Care Volume: 21 Issue: 1 Pages: 1-1
  • DOI: 10.1186/s13054-017-1811-1
  • NAID: 120006360078
  • Peer Reviewed / Open Access
• [Journal Article] Japan Septic Disseminated Intravascular Coagulation (JSEPTIC DIC) study group：Recombinant human soluble thrombomodulin and mortality in sepsis-induced disseminated intravascular coagulation. (2016)
  • Author(s): Hayakawa M, Yamakawa K, Saito S, Uchino S, Kudo D, Iizuka Y, Sanui M, Takimoto K, Mayumi T, Ono K.
  • Journal Title: Thrombosis and Haemostasis Volume: 115 Issue: 06 Pages: 1157-1166
  • DOI: 10.1160/th15-12-0987
  • Peer Reviewed / Open Access
• [Journal Article] Activated protein C does not increase in the early phase of trauma with disseminated intravascular coagulation: comparison with acute coagulopathy of trauma-shock. (2016)
  • Author(s): S. Jesmin, S. Gando, T. Wada, M. Hayakawa, A. Sawamura
  • Journal Title: Journal Intensive Care Volume: 4(4) Issue: 1 Pages: 1-1
  • DOI: 10.1186/s40560-015-0123-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Disseminated intravascular coagulation with the fibrinolytic phenotype predicts the outcome of patients with out-of-hospital cardiac arrest. (2016)
  • Author(s): Wada T, Gando S, Ono Y, Maekawa K, Katabami K, Hayakawa M, Sawamura A.
  • Journal Title: Thrombosis Journal Volume: 14 Issue: 1 Pages: 43-43
  • DOI: 10.1186/s12959-016-0116-y
  • Peer Reviewed / Open Access
• [Journal Article] Antithrombin Supplementation and Mortality in Sepsis-Induced Disseminated Intravascular Coagulation: A Multicenter Retrospective Observational Study. (2016)
  • Author(s): Hayakawa M, Kudo D, Saito S, Uchino S, Yamakawa K, Iizuka Y, Sanui M, Takimoto K, Mayumi T, Ono K, Azuhata T, Ito F, Yoshihiro S, Hayakawa K, Nakashima T, Ogura T, Noda E, Nakamura Y, Sekine R, Yoshikawa Y, Sekino M, Ueno K, Okuda Y, Watanabe M, Tampo A, Saito N, 他20名
  • Journal Title: Shock Volume: 46 Issue: 6 Pages: 623-631
  • DOI: 10.1097/shk.0000000000000727
  • NAID: 120005946857
  • Peer Reviewed / Open Access
• [Journal Article] Noble-Collip drum Trauma induces disseminated intravascular coagulation but not acute coagulopathy of trauma-shock. (2015)
  • Author(s): Hayakawa M, Gando S, Ono Y, Wada T, Yanagida Y, Sawamura A, Ieko M.
  • Journal Title: Shock Volume: 43 Issue: 3 Pages: 261-267
  • DOI: 10.1097/shk.0000000000000281
  • NAID: 120005712471
  • Peer Reviewed / Open Access
• [Journal Article] Fibrinogen level deteriorates before other routine coagulation parameters and massive transfusion in the early phase of severe trauma: a retrospective observational study. (2015)
  • Author(s): Hayakawa M, Gando S, Ono Y, Wada T, Yanagida Y, Sawamura A.
  • Journal Title: Seminars in Thrombosis and Hemostasis Volume: 41 Issue: 01 Pages: 35-42
  • DOI: 10.1055/s-0034-1398379
  • NAID: 120005693899
  • Peer Reviewed / Open Access
• [Journal Article] Rapid evaluation of fibrinogen levels using the CG02N whole blood coagulation analyzer. (2015)
  • Author(s): Hayakawa M, Gando S, Ono Y, Mizugaki A, Katabami K, Maekawa K, Miyamoto D, Wada T, Yanagida Y, Sawamura A.
  • Journal Title: Seminars in Thrombosis and Hemostasis Volume: 41 Issue: 03 Pages: 267-271
  • DOI: 10.1055/s-0035-1547372
  • NAID: 120005740322
  • Peer Reviewed / Open Access
• [Presentation] DIC and fibrinolysis in patients with trauma and out-of-hospital cardiac arbest (2016)
  • Author(s): Gando S, Wada T, Ono Y, Maekawa K, Katabami K, Hayakawa M, Sawamura A
  • Organizer: The 9th Congress of Asian-Pacific Society on Thrombosis and Hemostasis
  • Place of Presentation: Taipei International Convention Center (Taipei, Taiwan)
  • Year and Date: 2016-10-06
  • Int'l Joint Research / Invited
• [Presentation] Disseminated intravascular coagulation during eary phase of out-of-hospital cardiac arrest and resuscitation belongs to the fibrinolytic phonotype. (2016)
  • Author(s): Sadamoto Y, Katabami K, Wada T, Ono Y, Maekawa K, Hayakawa M, Sawamura A, Gando S.
  • Organizer: 29th Annual Congress of European Society of Intensive Care Medicine
  • Place of Presentation: MiCO Milano Congressi (Milano, Italy)
  • Year and Date: 2016-10-01
  • Int'l Joint Research
• [Presentation] isseminated intravascular coagulation with the fibrinolytic phenotype predicts the outcome of patients with patients with out-of-hospital cardiac arrest. (2016)
  • Author(s): Kodate A, Katabami K, Wada T, Ono Y, Maekawa K, Hayakawa M, Sawamura A, Gando S.
  • Organizer: 29th Annual Congress of European Society of Intensive Care Medicine
  • Place of Presentation: MiCO Milano Congressi (Milano, Italy)
  • Year and Date: 2016-10-01
  • Int'l Joint Research
• [Presentation] 「外傷性凝固障害－最新の知見－」外傷急性期のフィブリノゲン (2016)
  • Author(s): 早川峰司
  • Organizer: 第30回日本外傷学会総会・学術集会
  • Place of Presentation: 御茶ノ水ソラシティ（東京都千代田区）
  • Year and Date: 2016-05-30
• [Presentation] 外傷直後の凝固障害 ～なぜ線溶亢進型DICなのか～ (2015)
  • Author(s): 早川峰司
  • Organizer: Critical Care DIC Forum in OSAKA
  • Place of Presentation: ホテル阪急インターナショナル（大阪府大阪市）
  • Year and Date: 2015-09-26
  • Invited
• [Presentation] ラット鈍的外傷モデルにおける外傷直後の凝固傷害と凝固活性化因子 (2015)
  • Author(s): 早川峰司
  • Organizer: 第30回日本Shock学会総会
  • Place of Presentation: 京王プラザホテル八王子（東京都八王子市）
  • Year and Date: 2015-05-22