Hydrocortisone Therapy with CAR Peptide Protects the Injured Endothelial Glycocalyx in Sepsis.
Project/Area Number |
15K10973
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Emergency medicine
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Research Institution | Gifu University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
岡田 英志 岐阜大学, 医学部附属病院, 講師 (30402176)
竹村 元三 朝日大学, 歯学部, 教授 (40283311)
鈴木 浩大 岐阜大学, 医学部附属病院, 助教 (80724583)
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Research Collaborator |
SUGAHARA Kazuki
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 敗血症 / 血管内皮傷害 / ホーミングペプチド / 腎障害 / 血管内皮障害 |
Outline of Final Research Achievements |
A tissue-penetrating homing peptide, CARSKNKDC (CAR) has previously demonstrated the unique ability to facilitate transport of co-administered drugs into injured endothelial cell. Co-administration of hydrocortisone and CAR could improve the endothelial disorder and survival in sepsis using lower amounts of hydrocortisone.Mice were given lipopolysaccharide (LPS, 20 mg/kg). Subsequently, five different treatments were initiated: saline only; CAR peptide-alone administration 500 ug; hydrocortisone 0.2mg/kg or 10mg/kg ; and CAR co-administered with hydrocortisone 0.2mg/kg. Hydrocortisone and /or CAR peptide were injected intraperitoneally at 3, 12 and 24 hours after LPS administration. Forty-eight hours after LPS administration, combined thrapy group showed the best survival ratio. These results suggest that co-administration of hydrocortisone and CAR peptide is a highly effective treatment strategy for endothelial disorder in sepsis.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Three dimensional ultrastructure of capillary endothelial glycocalyx under normal and septic conditions.2017
Author(s)
Okada H, Takemura G, Suzuki K, Oda K, Takada C, Hotta Y, Miyazaki N, Tsujimoto A, Muraki I, Ando Y, Zaikokuji R, Matsumoto A, Kitagaki H, Tamaoki Y, Usui T, Doi T, Yoshida T, Yoshida S, Ushikoshi H, Toyoda I, Ogura S.
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Journal Title
Critical Care.
Volume: 26
Issue: 1
Pages: 261-261
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Neutrophil Elastase Plays a Pivotal Role in Sepsis-induced Myocardial Dysfunction2017
Author(s)
Kodai Suzuki, Hideshi Okada, Genzou Takemura, Fuminori Yamaji, Chihiro Takada, Kazumasa Oda, Yasuaki Hotta, Nagisa Miyazaki, Tomoaki Doi, Takahiro Yoshida, Shozo Yoshida, Hiroaki Ushikoshi, Shinji Ogura
Organizer
The 90th American Heart Association Scientific Sessions
Related Report
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[Presentation] Ulinastatin ameliorated acute kidney injury in sepsis via inhibition of endothelial injury2016
Author(s)
Akihiro Uchida, Hideshi Okada, Kodai Suzuki, Chihiro Takada, Airi Kageyama, Yuki Kato, Ayumi Kuroda, Yoshiaki Ogawa, Yasuaki Hotta, Taku Tanaka, Shiho Nakano, Takahiro Yoshida, Shozo Yoshida, Nagisa Miyazaki, Hiroaki Ushikoshi, Izumi Toyoda, Genzou Takemura, Shinji Ogura
Organizer
The 8th Congress of the International Federation of Shock Societies
Place of Presentation
Tokyo, Japan
Year and Date
2016-10-03
Related Report
Int'l Joint Research
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[Presentation] Low dosage aspirin attenuated multiple organ failure in sepsis through vascular endothelial protection2016
Author(s)
Takahiro Usui, Hideshi Okada, Kodai Suzuki, Chihiro Takada, Risa Inagawa, Go Furui, Hiroki Uekado, Hiroki Jinno, Junya Ota, Yasuaki Hotta, Taku Tanaka, Shiho Nakano, Takahiro Yoshida, Shozo Yoshida, Nagisa Miyazaki, Hiroaki Ushikoshi, Izumi Toyoda, Genzou Takemura, Shinji Ogura
Organizer
The 8th Congress of the International Federation of Shock Societies
Place of Presentation
Tokyo, Japan
Year and Date
2016-10-03
Related Report
Int'l Joint Research
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[Presentation] CAR Peptide EnhancesHydrocortisone to More Effectively Treat Injured Endothelium in Sepsis2016
Author(s)
Kodai Suzuki, Hideshi Okada, Chihiro Takada, Kazumasa Oda, Takahiro Yoshida, , Shiho Nakano, David Mann, Genzou Takemura, Masanobu Komatsu, Shinji Ogura
Organizer
The 8th Congress of the International Federation of Shock Societies
Place of Presentation
Tokyo, Japan
Year and Date
2016-10-03
Related Report
Int'l Joint Research
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[Presentation] Ultrastructural Analysis of Endothelial Disorder in Acute Kidney Injury by Sepsis2015
Author(s)
Kodai Suzuki, Hideshi Okada, Genzou Takemura, Yasuaki Hotta, Chihiro Takada, Shozo Yoshida, Hiroaki Ushikoshi, Izumi Toyoda, Shinji Ogura
Organizer
American Shock Society
Place of Presentation
Denver, CL, USA
Year and Date
2015-06-07
Related Report
Int'l Joint Research
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