| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
In order to clarify the transcriptional regulatory mechanisms of WDR35/naofen, a WD repeat (WDR)-containing protein, we cloned and functionally characterized the promoter region of the gene. The putative promoter activity was confirmed using dual luciferase reporter gene assay system. The minimal region required for basal activity of the promoter was determined by generating a series of deletion and point mutation constructs. The binding of a transcriptional factor EGR-1 to the minimal region was identified using electrophoretic mobility shift assay. Furthermore, binding activity of EGR-1 to the minimal region was confirmed using chromatin immunoprecipitation assay. We also showed that EGR-1 play an important role in modulating the expression of WDR35/naofen in Neuro2a cells. And bupivacaine induced the WDR35/naofen expression via enhancing EGR-1 activity.
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