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Elucidation of the mechanism of tooth eruption in DAP12 deficient and DAP12/FcRr deficient mice with impaired osteoclast differentiation using comprehensive gene expression analysis

Research Project

Project/Area Number 15K11002
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionTohoku University

Principal Investigator

Nakamura Megumi  東北大学, 歯学研究科, 助教 (20431512)

Co-Investigator(Kenkyū-buntansha) 笹野 泰之  東北大学, 歯学研究科, 教授 (30196191)
Co-Investigator(Renkei-kenkyūsha) TAKAI toshiyuki  
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordsDAP12 / FcRr / 破骨細胞分化 / 歯の萌出 / 大理石骨病 / c-fos / FcRγ
Outline of Final Research Achievements

In this study, the teeth and surrounding periodontal tissues in the process of eruption were morphologically examined in DAP12 deficient and DAP12/FcRr double deficient mice with normal tooth eruption in spite of inhibited osteoclast differentiation and c-fos deficient mice with failure of tooth eruption due to impaired osteoclast differentiation. Micro-CT and histological analyses showed that TRAP-positive cells were detected in the mandible of 2-week-old DAP12 deficient mice and mandibular bone volume in DAP12 deficient mice were similar to that in wild type mice at two weeks of age, while mandibular bone volume in 12-week-old DAP12/FcRr deficient mice were higher than wild type mice.
These data suggest that osteoclasts are present in the mandibular bone of DAP12 deficient and DAP12/FcRr deficient mice, and these mice may not exhibit osteopetrosis until they are one month old.

Academic Significance and Societal Importance of the Research Achievements

破骨細胞分化が抑制されたDAP12欠損マウスとDAP12/FcRr二重欠損マウスの顎骨・歯・歯周組織についての詳細な情報はなく、本研究が初めての報告となる。また、網羅的遺伝子解析によりDAP12とFcRrの欠損に対して代償性に働く分子が同定できる可能性がある。
従って、本研究により得られる結果は、破骨細胞分化に関与する新規の分子を特定し、破骨細胞分化の分子メカニズムの解明に貢献するとともに、複雑な歯の萌出メカニズムの一端を解明し、顎口腔領域の発生研究分野に有益な情報を提供できるものと考える。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (8 results)

All 2019 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (7 results) (of which Invited: 1 results)

  • [Journal Article] Comparison of the Performance of Cutting Bone and Soft Tissue between Piezosurgery and Conventional Rotary Instruments2018

    • Author(s)
      Otake Y, Nakamura M, Henmi A, Takahashi T, Sasano Y
    • Journal Title

      Scientific Reports

      Volume: Nov 21; 8(1) Issue: 1 Pages: 171548-171548

    • DOI

      10.1038/s41598-018-35295-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 凍結歯胚移植に伴う硬組織形成の形態学的解析2019

    • Author(s)
      中村恵、笹野泰之
    • Organizer
      第124回日本解剖学会総会・全国学術集会 シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Transplantation of cryopreserved mouse tooth germ2017

    • Author(s)
      Li Xinghan, Megumi Nakamura, Yasuyuki Sasano
    • Organizer
      第122回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] 凍結保存歯胚を移植すると、歯根が伸長し歯が萌出する2017

    • Author(s)
      中村 恵、笹野泰之
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] マウス下顎骨発生過程では破骨細胞は石灰化に先行して出現する2017

    • Author(s)
      青山直樹、中村 恵、笹野泰之
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 骨と軟組織に対するピエゾサージェリーの切削能に関する実験的検討2017

    • Author(s)
      大竹義雄、中村 恵、逸見晶子、高橋 哲、笹野泰之
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 歯胚移植における凍結保存の実験的応用2017

    • Author(s)
      中村 恵、笹野泰之
    • Organizer
      第62回日本口腔外科学会総会・学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 骨と軟組織の切削におけるピエゾサージェリーと回転切削器具の実験的比較検討2017

    • Author(s)
      大竹義雄、中村 恵、逸見晶子、笹野泰之、高橋 哲
    • Organizer
      第62回日本口腔外科学会総会・学術大会
    • Related Report
      2017 Research-status Report

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Published: 2015-04-16   Modified: 2020-03-30  

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