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The functional study of GABA and serotonin in differentiation of taste bud cells using Cre-loxP system

Research Project

Project/Area Number 15K11018
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionKyushu Dental College

Principal Investigator

Seta Yuji  九州歯科大学, 歯学部, 教授 (90291616)

Co-Investigator(Kenkyū-buntansha) 豊野 孝  九州歯科大学, 歯学部, 准教授 (10311929)
中富 満城  九州歯科大学, 歯学部, 講師 (10571771)
小野 堅太郎  九州歯科大学, 歯学部, 教授 (40316154)
片岡 真司  九州歯科大学, 歯学部, 助教 (80364149)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords味蕾 / 転写因子 / Mash1 / セロトニン / GABA / マウス
Outline of Final Research Achievements

In the present study, to begin to understand these mechanisms, we investigated the role of Mash1-positive cells in regulating adult taste bud cell differentiation through the loss of Mash1-positive cells using the Cre-loxP system. We found that the cells expressing type III cell markers; aromatic L-amino acid decarboxylase (AADC), carbonic anhydrase 4 (CA4), glutamate decarboxylase 67 (GAD67), neural cell adhesion molecule (NCAM), and synaptosomal-associated protein 25 (SNAP25); were significantly reduced in the circumvallate taste buds after administration of tamoxifen. However gustducin and Phospholipase C beta2 (PLC beta2);markers of type II taste bud cells; were not significantly changed in the circumvallate taste buds after administration of tamoxifen. These results suggest that Mash1-positive cells could be differentiated to the type III cells, not to the type II cells in the taste bud.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (20 results)

All 2018 2017 2016 2015 Other

All Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Open Access: 1 results) Presentation (11 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Journal Article] Hey1 and Hey2 are differently expressed during mouse tooth development.2018

    • Author(s)
      Kibe K, Nakatomi M, Kataoka S, Toyono T, Seta Y.
    • Journal Title

      Gene Expr Patterns.

      Volume: 27 Pages: 99-105

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 1.Lysophosphatidylcholine acyltransferase 4 is involved in chondrogenic differentiation of ATDC5 cells.2017

    • Author(s)
      Tabe S, Hikiji H, Ariyoshi W, Hashidate-Yoshida T, Shindou H, Shimizu T, Okinaga T, Seta Y, Tominaga K, Nishihara T.
    • Journal Title

      Sci Rep.

      Volume: 7 Issue: 1 Pages: 1-11

    • DOI

      10.1038/s41598-017-16902-4

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ADAM17 regulates TNF-α expression upon lipopolysaccharide stimulation in oral keratinocytes.2017

    • Author(s)
      Hirayama A, Awano S, Seta Y, Ansai T.
    • Journal Title

      Biomed Res.

      Volume: 38 Pages: 218-233

    • NAID

      130006865608

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of N-cadherin and cell surface molecules in the taste buds of mouse circumvallate papillae.2017

    • Author(s)
      Matsuyama K, Seta Y, Kataoka S, Nakatomi M, Toyono T, Kawamoto T.
    • Journal Title

      J. Oral Biosci.

      Volume: 59 Pages: 218-233

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of vascular nucleotide transporter in rat odontoblasts.2016

    • Author(s)
      keda E, Goto T, Gunjigake K, Kuroishi K, Udea M, Kataoka S, Toyono T, Nakatomi M, Seta Y, Kitamura C, Nishihara T, Kawamoto T
    • Journal Title

      Acta Histochem. Cytochem.

      Volume: 49 Pages: 21-28

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] A spatial association between odontomas and the gubemaculum tracts.2016

    • Author(s)
      da M, Miyamoto I, Nishida I, Tanaka T, Kito S, Seta Y, Yada N, Saeki K, Matsumoto-Takeda S, Wakasugi-Sato N, Habu M, Kodama M, Kokuryo S, Nishimura S, Matsuo K, Tominaga K, Yoshioka I, Maki K, Morimoto Y
    • Journal Title

      Oral Surg. Oral Med. Oral Pathol. Oral Radiol.

      Volume: 121 Pages: 91-95

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Asorin in compressed periodontal ligament cells inhibits bone formation.2016

    • Author(s)
      Ueda M, Goto T, Kuroishi KN, Gunjigake KK, Ikeda E, Kataoka S, Nakatomi M, Toyono T, Seta Y, Kawamoto T
    • Journal Title

      Arch. Oral Biol.

      Volume: 62 Pages: 86-92

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Muscle regulatory factors regulate T1R3 taste receptor expression.2015

    • Author(s)
      Kokabu S, Lowery JW, Toyono T, Seta Y, Hitomi S, Sato T, Enoki Y, Okubo M, Fukushima Y, Yoda T.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 468 Pages: 568-573

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Promoter analysis for mouse T1R1 amino acids (umami) receptor gene in C2C12 cells2017

    • Author(s)
      Toyono T, Hirata Y, Kataoka S, Nakatomi M, Seta Y
    • Organizer
      The 16th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 筋芽細胞株C2C12における、転写因子KLF2によるアミノ酸(うま味)受容体T1R1遺伝子の転写調節機構の解析2017

    • Author(s)
      豊野孝、平田祐基、片岡真司、中富満城、細川隆司、瀬田祐司
    • Organizer
      第122回日本解剖学会全国学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 2C12における、アミノ酸受容体T1R1遺伝子の転写調節機構の解析2017

    • Author(s)
      平田祐基、豊野孝、片岡真司、中富満城、細川隆司、瀬田祐司
    • Organizer
      九州歯科学会学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] マウス歯胚形成過程における低酸素負荷の影響2017

    • Author(s)
      木部琴乃、中富満城、片岡真司、豊野孝、瀬田祐司
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] DNAアフィニティ沈降法を用いたうま味(アミノ酸)受容体T1R1遺伝子の転写調節機構の解析2017

    • Author(s)
      豊野孝、平田祐基、片岡真司、中富満城、瀬田祐司
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] マウス味蕾における膜表面分子の発現2016

    • Author(s)
      松山佳永、川元龍夫、瀬田祐司
    • Organizer
      第76回九州歯科学会学術総会
    • Place of Presentation
      北九州
    • Year and Date
      2016-05-28
    • Related Report
      2016 Research-status Report
  • [Presentation] Mash1による味蕾細胞分化制御2016

    • Author(s)
      高木拓樹、川元龍夫、瀬田祐司
    • Organizer
      第76回九州歯科学会学術総会
    • Place of Presentation
      北九州
    • Year and Date
      2016-05-28
    • Related Report
      2016 Research-status Report
  • [Presentation] マウス味蕾における膜表面分子の発現2016

    • Author(s)
      松山佳永、川元龍夫、瀬田祐司
    • Organizer
      第58回日本顕微鏡学会九州支部学術講演会
    • Place of Presentation
      北九州
    • Related Report
      2016 Research-status Report
  • [Presentation] Cre-loxPシステムを利用した味蕾Ⅲ型細胞分化におけるMash1の機能解析2016

    • Author(s)
      高木拓樹、川元龍夫、瀬田祐司
    • Organizer
      第58回日本顕微鏡学会九州支部学術講演会
    • Place of Presentation
      北九州
    • Related Report
      2016 Research-status Report
  • [Presentation] Mash1による味蕾細胞の分化制御2015

    • Author(s)
      瀬田祐司
    • Organizer
      日本顕微鏡学会 九州支部総会
    • Place of Presentation
      福岡
    • Year and Date
      2015-11-21
    • Related Report
      2015 Research-status Report
  • [Presentation] The functional analysis of Mash1 in mouse taste bud cell differentiation using Cre-loxP system.2015

    • Author(s)
      Seta Y, Toyono T, Kataoka S, Nakatomi M
    • Organizer
      The 13th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception.
    • Place of Presentation
      Fukuoka
    • Year and Date
      2015-11-03
    • Related Report
      2015 Research-status Report
  • [Remarks] 九州歯科大学 解剖学分野

    • URL

      http://www2.kyu-dent.ac.jp/depart/2kaibou/Site/HOME.html

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-12-27  

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