The functional study of GABA and serotonin in differentiation of taste bud cells using Cre-loxP system
| Project/Area Number |
15K11018
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
Seta Yuji 九州歯科大学, 歯学部, 教授 (90291616)
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| Co-Investigator(Kenkyū-buntansha) |
豊野 孝 九州歯科大学, 歯学部, 准教授 (10311929)
中富 満城 九州歯科大学, 歯学部, 講師 (10571771)
小野 堅太郎 九州歯科大学, 歯学部, 教授 (40316154)
片岡 真司 九州歯科大学, 歯学部, 助教 (80364149)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 味蕾 / 転写因子 / Mash1 / セロトニン / GABA / マウス |
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| Outline of Final Research Achievements |
In the present study, to begin to understand these mechanisms, we investigated the role of Mash1-positive cells in regulating adult taste bud cell differentiation through the loss of Mash1-positive cells using the Cre-loxP system. We found that the cells expressing type III cell markers; aromatic L-amino acid decarboxylase (AADC), carbonic anhydrase 4 (CA4), glutamate decarboxylase 67 (GAD67), neural cell adhesion molecule (NCAM), and synaptosomal-associated protein 25 (SNAP25); were significantly reduced in the circumvallate taste buds after administration of tamoxifen. However gustducin and Phospholipase C beta2 (PLC beta2);markers of type II taste bud cells; were not significantly changed in the circumvallate taste buds after administration of tamoxifen. These results suggest that Mash1-positive cells could be differentiated to the type III cells, not to the type II cells in the taste bud.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Expression of vascular nucleotide transporter in rat odontoblasts.2016
Author(s)
keda E, Goto T, Gunjigake K, Kuroishi K, Udea M, Kataoka S, Toyono T, Nakatomi M, Seta Y, Kitamura C, Nishihara T, Kawamoto T
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Journal Title
Acta Histochem. Cytochem.
Volume: 49
Pages: 21-28
Related Report
Peer Reviewed
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[Journal Article] A spatial association between odontomas and the gubemaculum tracts.2016
Author(s)
da M, Miyamoto I, Nishida I, Tanaka T, Kito S, Seta Y, Yada N, Saeki K, Matsumoto-Takeda S, Wakasugi-Sato N, Habu M, Kodama M, Kokuryo S, Nishimura S, Matsuo K, Tominaga K, Yoshioka I, Maki K, Morimoto Y
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Journal Title
Oral Surg. Oral Med. Oral Pathol. Oral Radiol.
Volume: 121
Pages: 91-95
Related Report
Peer Reviewed
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