Fundamental study on delivery system of DNA vaccine-embedded CPH nanoparticles for cancer prevention using oral mucosa

• Project/Area Number: 15K11031
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Aichi Gakuin University
• Principal Investigator: MAEDA HATSUHIKO 愛知学院大学, 歯学部, 教授 (30175591)
• Co-Investigator(Kenkyū-buntansha): 久保 勝俊 愛知学院大学, 歯学部, 准教授 (60329604)
• Co-Investigator(Renkei-kenkyūsha): SUGITA Yoshihiko 愛知学院大学, 歯学部, 准教授 (20367618) ; SATO Emiko 愛知学院大学, 歯学部, 講師 (60111001) ; YOSHIDA Waka 愛知学院大学, 歯学部, 講師 (10513210)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: DNAワクチン / パピローマウイルス / ナノスフェアー / CPHナノ粒子 / テープストリッピング法 / ドラッグデリバリー / 口腔 / 癌抑制効果 / ナノ粒子 / 癌化抑制効果 / 扁平上皮癌 / naked pDNA

Outline of Final Research Achievements

The delivery system of DNA vaccine-embedded hydrophobic pullulan nanoparticles (CPH nanoparticles) with tape stripping method using oral mucosa for cancer prevention, that associated papillomavirus infection, was studied.
As a result, it was suggested that DNA vaccine-embedded CPH nanoparticles with tape stripping method was useful for cancer prevention. Therefore, it contributes to the papillomavirus-induced oral cancer prevention.

Research Products

• [Journal Article] Enhancement of DNA Vaccine Potency Against Hamster Oral Papillomavirus-Associated Oral Cancer by Electroporation <i>in vivo</i> (2017)
  • Author(s): Masato Jinno, Madoka Isomura, Nobuaki Sato, Yasuyoshi Torii, Waka Yoshida, Yoshihiko Sugita, Katsutoshi Kubo, Hatsuhiko Maeda
  • Journal Title: Journal of Hard Tissue Biology Volume: 26 Issue: 2 Pages: 127-134
  • DOI: 10.2485/jhtb.26.127
  • NAID: 130006832110
  • ISSN: 1341-7649, 1880-828X
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Human Papillomavirus Type 16 Infection and Local Lymph Node Metastasis in Patients with Squamous Cell Carcinoma (2016)
  • Author(s): Ryoko Kawai, Madoka Isomura, Nobuaki Sato, Seeta Kato, Waka Yoshida, Kei Kamiya, Yoshitaka Nagaya, Tomofumi Hattori, Koji Suzuki, Akiyoshi Funato, Masanori Yoshiyama, Yoshihiko Sugita, Katsutoshi Kubo, Hatsuhiko Maeda
  • Journal Title: Jounal of Hard Tissue Biology Volume: 25 Pages: 115-126
  • NAID: 130005145111
  • Peer Reviewed / Acknowledgement Compliant